菌物学报 ›› 2021, Vol. 40 ›› Issue (10): 2843-2853.doi: 10.13346/j.mycosystema.210188

• 简报 • 上一篇    下一篇

梵净山山顶苔藓矮林AM真菌多样性

何荣健,明燕,姚莉梅,朱青青,江龙()   

  1. 贵州大学生命科学学院 山地植物资源保护与保护种质创新教育部重点实验室 贵州 贵阳 550025
  • 收稿日期:2021-05-16 接受日期:2021-06-28 出版日期:2021-10-22 发布日期:2021-11-05
  • 通讯作者: 江龙 E-mail:ljiang@gzu.edu.cn
  • 作者简介:ORCID: HE Rong-Jian (0000-0002-2767-8263)
  • 基金资助:
    国家科技支撑项目(2015BAD04B0204);贵州梵净山国家级自然保护区管理局项目(梵管局[科])(2019-GD-AM)

Diversity of arbuscular mycorrhizal fungi in mossy dwarf forest of Fanjing Mountain, Guizhou Province, Southwest China

HE Rong-Jian,MING Yan,YAO Li-Mei,ZHU Qing-Qing,JIANG Long()   

  1. College of Life Sciences, Guizhou University, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guiyang, Guizhou 550025, China
  • Received:2021-05-16 Accepted:2021-06-28 Online:2021-10-22 Published:2021-11-05
  • Contact: Long JIANG E-mail:ljiang@gzu.edu.cn
  • Supported by:
    National Key Technology Research and Development Program of China(2015BAD04B0204);Guizhou Fanjing Mountain National Nature Reserve Administration Project (FGJ2019-GD-AM).(2019-GD-AM)

摘要:

为探析和比较梵净山国家自然保护区矮林生态系统中丛枝菌根(arbuscular mycorrhizal,AM)真菌群落特征,对6种山顶苔藓矮林群落(山樱-山矾Prunus-Symplocos、杜鹃-槭树Rhododendron-Acer、花楸-杜鹃Sorbus-Rhododendron、槭树Acer、黔稠Cyclobalanopsis stewardiana、杜鹃Rhododendron)土壤进行AM真菌分离、鉴定和分析。结果表明:梵净山山顶苔藓矮林群落共有13属32种AM真菌,包括无梗囊霉属Acaulospora 13种、球囊霉属Glomus 5种、近明球囊霉属Claroideoglomus 2种、多样孢囊霉属Diversispora 2种、隔球囊霉属Septoglomus 2种、原囊霉属Archaeospora 1种、双型囊霉属Ambispora 1种、内养囊霉属Entrophospora 1种、和平囊霉属Pacispora 1种、硬囊霉属Sclerocystis 1种、西维丁囊霉属Sieverdingia 1种、盾巨孢囊霉属Scutellospora 1种和巨孢囊霉属Gigaspora 1种,其中无梗囊霉属的分离频率、相对多度和重要值最高,分别是100%、65.90%和82.95%,为矮林区域的优势属,柯氏无梗囊霉为该区域的优势种;不同矮林间AM真菌群落结构组成差异显著,槭树矮林的孢子密度、菌根侵染率和Shannon-Wiener指数显著高于其他5种植物群落,黔稠矮林的孢子密度和物种丰富度最低,杜鹃矮林的AM真菌Shannon-Wiener指数和Simpson指数均为所有植物群落中最低,表明不同矮林类型对AM真菌群落结构具有重要影响。

关键词: 真菌群落结构, 山顶苔藓矮林, 多样性指数

Abstract:

The characteristics of arbuscular mycorrhizal (AM) fungal community of the mossy dwarf forest in Fanjingshan National Nature Reserve were explored, and AM fungal species from six different community types of top-montane dwarf mossy forest (Prunus-Symplocos, Rhododendron-Acer, Sorbus-Rhododendron, Acer, Cyclobalanopsis stewardiana and Rhododendron) were isolated and identified. In total, 32 species in 13 genera of AM fungi were isolated in the soil, including species of Acaulospora (13), Glomus (5), Claroideoglomus (2), Diversispora (2), Septoglomus (2), Archaeospora (1), Ambispora (1), Entrophospora (1), Pacispora (1), Sclerocystis (1), Sieverdingia (1), Scutellospora (1) and Gigaspora (1). The isolation frequency (100%), relative abundance (65.90%) and important value (82.95%) of Acaulospora were the highest, showing Acaulospora was the dominant genus in which Ac. koskei was the dominant species. As compared with other five dwarf forests, the spore density, root colonization rate and Shannon-Wiener index of soil AM fungi in Acer community were significantly higher. On the contrary, the spore density and species richness were the lowest in Cyclobalanopsis stewardiana community, and Shannon-Wiener index and Simpson index of AMF in Rhododendron community showed the lowest as compared with those in other dwarf forest communities. In conclusion, the community structure of AMF were incredibly influenced by environmental factors of different types of the mossy dwarf forest communities.

Key words: fungal community structure, mossy dwarf forest, diversity index