菌物学报 ›› 2021, Vol. 40 ›› Issue (10): 2811-2820.doi: 10.13346/j.mycosystema.210216

• 研究论文 • 上一篇    下一篇

褐环乳牛肝菌对樟子松生长的调控——影响激素和代谢产物含量

尹大川(),祁金玉   

  1. 沈阳农业大学 辽宁 沈阳 110866
  • 收稿日期:2021-06-07 接受日期:2021-07-06 出版日期:2021-10-22 发布日期:2021-11-05
  • 通讯作者: 尹大川 E-mail:yindachuan@syau.edu.cn
  • 基金资助:
    辽宁省博士科研启动基金计划(2019-BS-208);国家自然科学基金(31800542)

Regulation of Suillus luteus on the growth of Pinus sylvestris var. mongolica: influencing the hormone and metabolite content of the seedlings

YIN Da-Chuan(),QI Jin-Yu   

  1. Shenyang Agricultural University, Shenyang, Liaoning 110866, China
  • Received:2021-06-07 Accepted:2021-07-06 Online:2021-10-22 Published:2021-11-05
  • Contact: Da-Chuan YIN E-mail:yindachuan@syau.edu.cn
  • Supported by:
    Doctoral Research Initiation Fund of Liaoning Province(2019-BS-208);National Natural Science Foundation of China(31800542)

摘要:

为了进一步探究褐环乳牛肝菌对樟子松苗木生长的促进机制,利用生物化学和植物生理学等研究方法,在前期研究基础上对2年生沙地樟子松苗木施加褐环乳牛肝菌液体菌剂,并在2个月后对苗木各部分生长状态、生长相关激素、抗逆性代谢产物进行检测。结果表明:褐环乳牛肝菌可以通过升高樟子松IAA和GA含量促进樟子松苗木的生长。其中,对樟子松根系中IAA含量影响最为显著,接菌苗木根中IAA含量比未接菌苗木根中IAA含量高85%以上;与此结果相反,褐环乳牛肝菌可以降低樟子松苗木ABA含量,通过ABA含量变化调控IAA含量,进而调控根系的生长;除此之外,褐环乳牛肝菌可以调控樟子松苗木甜菜碱和黄酮化合物的含量。未接菌苗木的甜菜碱在根、茎、叶中的含量分别比接菌苗木高46%、26%和23%,说明接菌苗木在受环境的影响方面相较于未接菌苗木低。接菌处理可以显著提高樟子松苗木根部黄酮化合物含量。本研究结果可以为今后进一步研究提供数据借鉴,同时也可以为辽西半干旱地区森林健康调控与褐环乳牛肝菌科学应用于当地造林提供科学依据。

关键词: 褐环乳牛肝菌, 樟子松, 植物激素, 促生机制, 生长调控

Abstract:

On the basis of previous studies, the growth status, growth-related hormones and stress-resistant metabolites of various parts of 2-year-old sandy P. sylvestris var. mongolica seedlings were detected in two months after applying liquid inoculant of S. luteus for further exploring the promotion mechanism of Suillus luteus on the growth of Pinus sylvestris var. mongolica seedlings. Results showed that S. luteus could promote the growth of P. sylvestris var. mongolica by increasing the content of IAA and GA. IAA content in the roots was most significantly affected, and the content in inoculated seedlings was 85% higher than that in uninoculated ones. On the contrary, S. luteus could reduce the ABA content, and the content of IAA was regulated by variation of ABA content, resulting in regulation of root growth. In addition, S. luteus could regulate the content of betaine and flavonoids. The content of betaine in roots, stems and leaves of uninoculated seedlings was 46%, 26% and 23% higher than that of inoculated ones, indicating that inoculated seedlings were less affected by the environment as compared with uninoculated ones. The content of flavonoids in roots was significantly increased after inoculation with S. luteus inoculant. Results of this study provide reference for forest health regulation and scientific application of S. luteus in local afforestation in the semi-arid area of western Liaoning Province.

Key words: Suillus luteus, Pinus sylvestris var. mongolica, plant hormone, growth-promoting mechanism, growth control