菌物学报 ›› 2021, Vol. 40 ›› Issue (10): 2800-2810.doi: 10.13346/j.mycosystema.210222

• 研究论文 • 上一篇    下一篇

丛枝菌根真菌调控根系构型与矿质元素平衡提高西瓜植株耐盐性的研究

王策1,2,谢宏鑫1,2,刘润进1,李伟1,郭绍霞1,李敏1,2,*()   

  1. 1.青岛农业大学菌根生物技术研究所 山东 青岛 266109
    2.青岛农业大学园艺学院 山东 青岛 266109
  • 收稿日期:2021-06-11 接受日期:2021-07-14 出版日期:2021-10-22 发布日期:2021-11-05
  • 通讯作者: 李敏 E-mail:minli@qau.edu.cn
  • 作者简介:ORCID: WANG Ce (0000-0002-9022-7799)
  • 基金资助:
    山东省重大科技创新项目(2019JZZY010715);山东省重点研发计划(2019GNC106043)

Salt tolerance of watermelon plants through AM fungus adjusting root architecture and mineral element balance

WANG Ce1,2,XIE Hong-Xin1,2,LIU Run-Jin1,LI Wei1,GUO Shao-Xia1,LI Min1,2,*()   

  1. 1. Institute of Mycorrhizal Biotechnology, Agricultural University of Qingdao, Qingdao, Shandong 266109, China
    2. College of Horticulture, Agricultural University of Qingdao, Qingdao, Shandong 266109, China
  • Received:2021-06-11 Accepted:2021-07-14 Online:2021-10-22 Published:2021-11-05
  • Contact: Min LI E-mail:minli@qau.edu.cn
  • Supported by:
    Major Scientific Innovation Project of Shandong Province(2019JZZY010715);Key Technology Research and Development of Shandong Province(2019GNC106043)

摘要:

丛枝菌根(arbuscular mycorrhiza,AM)真菌可通过多种途径或机制来增强植物的耐盐性,进而促进植株的生长发育。本研究在盆栽条件下设西瓜Citrullus lanatus品种‘京欣四号’幼苗接种变形球囊霉Glomus versiforme和不接种以及施加和不施加100mmol/L NaCl共4个处理,测定植株根系菌根侵染状况、根系构型及其根茎叶中钾(K)、钙(Ca)、磷(P)、钠(Na)含量、K+/Na+、Ca2+/Na和植株生长状况等。AM真菌显著增加了盐胁迫下西瓜植株总根长度、根表面积、根体积和根尖数量,改善了根系构型;促进了西瓜根系对K、Ca和P的吸收,提高了茎Ca和P含量、根系K和P含量、K+/Na+和Ca2+/Na+,而降低了根Na+含量;茎P和Na+、叶K和Ca的含量显著高于其他器官相应含量。典范对应分析表明,根系K含量、K+/Na+和Ca2+/Na+与总根长度、主根长度、根表面积、根体积、根尖数量、根平均直径呈正相关;叶K+/Na+与主根长度呈正相关;根系Na+含量与根系总根长、根平均直径和根尖数量呈负相关。接种AM真菌改善了矿质元素平衡及其分配状况。盐胁迫后西瓜植株对菌根的依赖性增强。结果表明,K、Ca、P是AM真菌介导植物耐盐性的关键养分;K+/Na+和Ca2+/Na+是重要的矿质元素平衡指标,接种AM真菌能调控植物根系构型和矿质元素平衡状况,从而缓解盐胁迫对西瓜生长的抑制作用,提高植株的耐盐性。

关键词: 盐胁迫, 丛枝菌根真菌, 西瓜, 矿质元素

Abstract:

Arbuscular mycorrhizal (AM) fungi can enhance the salt tolerance of plants and promote the growth and development of plants through various mechanisms. In this study, seedlings of watermelon Citrullus lanatus ‘Jingxin 4’ were inoculated with AM fungus Glomus versiforme, and applied with 100mmol/L NaCl solution to determine the mycorrhizal colonization status, root architecture, the content of potassium (K), calcium (Ca), phosphorus (P) and sodium (Na) in roots, stems and leaves, the ratio of K +/Na+ and Ca2+/Na+, and the growth of pot plants under chamber conditions. The results showed that AM fungi significantly increased the total root length, root surface area, root volume and root tip number of watermelon plants under salt stress, and improved root architecture characteristics, promoted the absorption of K, Ca and P, increased the content of Ca and P in stem, of K and P in root, and the ratio of K+/Na+ and Ca2+/Na+, but decreased the content of Na+ in root. The content of P and Na+ in stem, and of K and Ca in leaves was significantly higher than that in other organs. Canonical correspondence analysis showed that root K content, K+/Na+ and Ca2+/Na+ were significantly positively correlated with total root length, taproot length, root surface area, root volume, root tip number, and average diameter of roots. Leaf K+/Na+ was positively correlated with taproot length. The root Na+ content was negatively correlated with the total root length, average root diameter and the number of root tips. The equilibrium and distribution of mineral elements were improved by AM fungi. The dependence of watermelon plants on mycorrhiza was enhanced after salt stress. It is concluded that K, Ca and P are the key nutrients for AM fungus-mediating salt tolerance in plants, while K+/Na+ and Ca2+/Na+ are important mineral element equilibrium indexes. AM fungal inoculation can regulate the root architecture and mineral element balance of the plant, thus alleviating the inhibition effect of salt stress on the growth of watermelon and improving the salt tolerance of the plant.

Key words: salt stress, arbuscular mycorrhizal fungi, watermelon, mineral elements