菌物学报 ›› 2021, Vol. 40 ›› Issue (10): 2700-2715.doi: 10.13346/j.mycosystema.210269

• 研究论文 • 上一篇    下一篇

中国江西龙虎山崖顶植物根中深色有隔内生菌多样性

张红芳1,2,何刚1,2,孙启彪1,2,季晓红1,2,欧阳建萍1,2,何贞英3,康美如2,陈晔1,2,*()   

  1. 1.九江学院九江市真菌资源保育及应用重点实验室 江西 九江 332000
    2.九江学院药学与生命科学学院 江西 九江 332000
    3.九江学院电子商务学院 江西 九江 332005
  • 收稿日期:2021-07-15 接受日期:2021-08-11 出版日期:2021-10-22 发布日期:2021-11-05
  • 通讯作者: 陈晔 E-mail:chenyejjtc@126.com
  • 基金资助:
    江西省自然科学基金(20171BAB204007);江西省教育厅科技项目(GJJ190912);江西省教育厅科技项目(GJJ190925);江西省教育厅科技项目(GJJ190940)

Diversity of dark septate endophytes in cliff-top plant roots in Longhu Mountain, Jiangxi Province, East China

ZHANG Hong-Fang1,2,HE Gang1,2,SUN Qi-Biao1,2,JI Xiao-Hong1,2,OUYANG Jian-Ping1,2,HE Zhen-Ying3,KANG Mei-Ru2,CHEN Ye1,2,*()   

  1. 1. Jiujiang Key Laboratory of Fungal Resources Conservation and Utilization, Jiujiang University, Jiujiang, Jiangxi 332000, China
    2. College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, Jiangxi 332000, China
    3. College of Electronic Commerce, Jiujiang University, Jiujiang, Jiangxi 332005, China
  • Received:2021-07-15 Accepted:2021-08-11 Online:2021-10-22 Published:2021-11-05
  • Contact: Ye CHEN E-mail:chenyejjtc@126.com
  • Supported by:
    Jiangxi Provincial Natural Science Foundation(20171BAB204007);Scientific Research Project of Jiangxi Provincial Education Department(GJJ190912);Scientific Research Project of Jiangxi Provincial Education Department(GJJ190925);Scientific Research Project of Jiangxi Provincial Education Department(GJJ190940)

摘要:

为探明丹霞地貌区崖顶植物深色有隔内生真菌(dark septate endophyte,DSE)多样性、群落组成以及生态分布规律。本文以江西龙虎山崖顶常见植物刺柏、马尾松、青冈栎、檵木、乌饭树、鸭跖草、苦槠、辣木树和香附子9种植物的根为研究对象,采用经典的组织分离方法分离DSE真菌,结合形态学特征和分子生物学数据研究丹霞地貌区崖顶常见植物DSE真菌多样性。结果表明:从9种植物根部990个组织块中,共分离纯化出404株菌株,经鉴定隶属于45个分类单元。其中,青霉属Penicillium、拟内孢霉属Endomycopsis、曲霉属Aspergillus、头囊霉属Ascocybe为优势属群,属种组成在不同植物中存在差异,且内生真菌属的数目与植物优势度极显著正相关(P<0.01)。9种植物深色有隔内生真菌(DSE)总分离率在0.51%-13.33%之间;各植物DSE Shannon指数在0.0743-1.0400之间,与植物优势度极显著正相关(P<0.01),差异明显;Simpson指数较高(均值>0.7)且Pielou指数较低(均值<0.4)表明DSE真菌在不同植物分布不均匀;相似性指数普遍不高,在0.071-0.467之间。本研究初步揭示了丹霞地貌区崖顶深色有隔内生真菌的分布及多样性情况,为进一步探讨内生真菌在丹霞地貌等特殊生境的生态功能提供参考。

关键词: 丹霞地貌, 深色有隔内生真菌, 多样性指数, 相似性指数

Abstract:

Dark septate endophytes (DSEs) are typical plant symbiotic fungi, playing an important role in ecosystem function. The diversity, community composition, and ecological patterns of DSEs in cliff-top plants in Danxia landform area are still unclear. In this study, the community composition of DSEs in the roots of nine dominant xerophytic plant species, including Castanopsis sclerophylla, Commelina communis, Cyclobalanopsis glauca, Cyperus rotundus, Juniperus formosana, Loropetalum chinensis, Moringa oleifera, Pinus massoniana and Vaccinium bracteatum in Longhu Mountain, Yingtan, China, was investigated. DSEs were isolated using tissue separation method and identified based on morphological and molecular methods. A total of 404 isolates belonging to 45 taxa was isolated from 990 tissue blocks. Of these, Penicillium, Endomycopsis, Aspergillus, and Ascocybe were the dominant genera. DSE community composition distinctly differed among plant species. The richness of genera was positively correlated with plant dominance. The isolation rate of DSEs of respective plant spieces ranged from 0.5% to 13.3%. However, the richness of DSEs in different plant species was relatively low. The Shannon index of DESs in each plant ranged from 0.07 to 1.04, showing positive correlation with plant dominance. The high Simpson diversity indices (avg.>0.7) and the low Pielou evenness indices (avg.<0.4) indicated the heterogeneous distribution of DSEs among plant species. The similarity index among DSE communities ranged from 0.07 to 0.47, showing different community composition across plant species. This study reveals the distribution and diversity of DSEs in cliff-top vegetation of Danxia landforms, which benefits to excavate and utilize the resource of DSEs.

Key words: Danxia landform, dark septate endophyte, diversity index, similarity coefficient