菌物学报 ›› 2014, Vol. 33 ›› Issue (4): 838-846.doi: 10.13346/j.mycosystema.130116

• 论文 • 上一篇    下一篇

蓝光光照对蛹虫草子实体生长和主要活性成分的影响

廉添添1,2,董彩虹3,杨涛3,4,孙军德5   

  1. 1沈阳农业大学土地与环境学院 辽宁 沈阳 110866
    2中国科学院微生物研究所真菌学国家重点实验室 北京 100101
    3中国科学院微生物研究所真菌学国家重点实验室 北京 100101
    4中国科学院大学 北京 100049
    5沈阳农业大学土地与环境学院 辽宁 沈阳 110866
  • 出版日期:2014-08-15 发布日期:2014-07-15
  • 基金资助:
    国家自然科学基金(No. 31100014);国家重点基础研究发展计划(No. 2014CB138302);中国科学院微生物研究所真菌学国家重点实验室资助

Effects of blue light on the growth and bioactive compound production of Cordyceps militaris

LIAN Tian-Tian1,2,DONG Cai-Hong3,YANG Tao3,4,SUN Jun-De5   

  1. 1College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning 110866,
    2China State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    3State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101,
    4China University of Chinese Academy of Sciences, Beijing 100049, China
    5College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning 110866, China
  • Online:2014-08-15 Published:2014-07-15

摘要: 光作为一种重要的环境因子,可影响真菌的生长发育、生理周期、形态变化及次级代谢产物的产生,对于蛹虫草而言,光照还是其子实体生长发育的必要条件。选择2株不同来源的蛹虫草菌株,研究了自然光照和蓝光光照条件下,其子实体生长、抗氧化酶活性和主要活性成分的变化。结果表明蓝光光照对于蛹虫草子实体产量没有明显的促进和抑制作用。自然光照条件下成熟子实体的超氧化物歧化酶(superoxide dismutase,SOD)活性显著高于蓝光光照,过氧化氢酶(catalase,CAT)活性没有明显差别,而过氧化物酶(peroxidase,POD)活性则表现出菌株差异。子实体主要活性成分方面,腺苷、甘露醇含量不受蓝光影响,类胡萝卜素在蓝光光照条件下含量极显著高于自然光光照,蓝光对子实体、虫草素、粗多糖含量的影响则存在菌株差异。研究为蛹虫草子实体的栽培条件优化,提高蛹虫草子实体质量提供依据。

关键词: 蛹虫草, 蓝光, 抗氧化酶, 腺苷, 虫草素, 类胡萝卜素

Abstract: Light as an important environmental factor can affect growth, life cycles, morphological characters and secondary metabolism of fungi. Light is a necessary environmental factor for the stroma formation and development of Cordyceps militaris. Effects of blue light on the growth of fruit body, anti-oxidative enzyme activities and bioactive compounds in two different strains, strain 85 and strain 138, of C. militaris were studied. It was indicated that blue light could not increase the superoxide dismutase (SOD) activities but could increase the content of carotenoids significantly of C. militaris. There were no significant differences in the yield of fruit bodies, catalase (CAT) activity and the content of adenosine and mannitol under blue and natural illuminations. The influences of blue light on the peroxidase (POD) activities, polysaccharide content and cordycepin content were different in the two strains.

Key words: Cordyceps militaris, blue light, anti-oxidative enzyme, adenosine, cordycepin, carotenoids