菌物学报 ›› 2019, Vol. 38 ›› Issue (11): 1965-1975.doi: 10.13346/j.mycosystema.190221

• 研究论文 • 上一篇    下一篇

氮和土著AMF对黄瓜间作土壤酶活性及氮利用的影响

刘圆圆1,2,张丽1,2,王硕1,刘大会3,张乃明1,2,杨锦花1,夏运生1,2,*()   

  1. 1. 云南农业大学资源与环境学院 云南 昆明 650201
    2. 云南省土壤培肥与污染修复工程实验室 云南 昆明 650201
    3. 湖北中医药大学药学院 湖北 武汉 430065
  • 收稿日期:2019-06-22 接受日期:2019-10-17 出版日期:2019-11-22 发布日期:2019-12-14
  • 通讯作者: 夏运生 E-mail:yshengxia@163.com
  • 作者简介:夏运生,中国农业大学资源环境生物技术专业博士,云南农业大学教授、硕士生导师及“百名”青年学术和技术带头人,主要从事菌根与农业污染控制研究。主持及完成国家自然科学基金、国家重点研发计划项目子课题等纵向和横向项目10余项,发表论文60多篇(其中SCI论文8篇,EI论文6篇)、授权专利9项、出版著作教材等8部、获省部级自然科学二等奖等科技奖励7项和云南省引进高层次人才享受政府购房补贴和工作经费资助。
  • 基金资助:
    国家自然科学基金(41561057);云南省重大科技专项?绿色食品国际合作研究中心专项课题(2019ZG00907-01);云南省土壤资源利用与保护创新团队项目(2015HC018)

Effects of nitrogen and inoculation of indigenous arbuscular mycorrhizal fungi on soil enzyme activity and nitrogen utilization of cucumber under intercropping conditions

Yuan-Yuan LIU1,2,Li ZHANG1,2,Shuo WANG1,Da-Hui LIU3,Nai-Ming ZHANG1,2,Jin-Hua YANG1,Yun-Sheng XIA1,2,*()   

  1. 1. College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan 650201, China
    2. Yunnan Soil Fertilization and Pollution Remediation Engineering Laboratory, Kunming, Yunnan 650201, China
    3. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China
  • Received:2019-06-22 Accepted:2019-10-17 Online:2019-11-22 Published:2019-12-14
  • Contact: Yun-Sheng XIA E-mail:yshengxia@163.com
  • Supported by:
    Supported by the National Natural Science Foundation of China(41561057);Special Project of International Cooperation and Research Center for Green Food in Yunnan Province(2019ZG00907-01);and Innovation Team Project of Utilization and Protection of Soil Resources in Yunnan Province(2015HC018)

摘要:

设施土壤氮(N)肥的大量不合理施用和高残留是导致作物硝态N含量超标和农业面源污染的主要因素之一。研究土著丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)与间作体系强化蔬菜对不同形态N的利用并结合土壤酶活性的反馈作用,可为设施土壤N素的高效利用和降低土壤N残留提供依据。本研究采用盆栽试验,设置黄瓜单作和黄瓜//大豆间作种植模式,不同AMF处理[不接种(NM)、接种土著AMF]和不同形态N处理[不施N(N0)、有机N(谷氨酰胺120mg/kg,ON120)、无机N(碳酸氢铵120mg/kg,ION120)],探讨了设施条件下施用不同形态N、接种土著AMF与间作大豆对黄瓜根围土壤酶活性及氮利用的影响。结果表明,与NM相比,接种土著AMF使设施黄瓜地上部、根系生物量及植株N吸收量均有不同程度的增加,根围土壤NH4 +-N、NO3 --N含量呈现降低趋势。同一N处理-土著AMF条件下,间作大豆处理下的黄瓜根系菌根侵染率显著高于单作处理;间作大豆也使黄瓜植株地上部、根系生物量及N吸收量显著增加,同时显著降低了根围土壤铵态N含量。此外,间作-土著AMF条件下,ON120和ION120处理的黄瓜根围土壤脲酶活性较N0处理分别提高了30%和14%,蛋白酶和硝酸还原酶活性也呈现出相同趋势。可见,所有复合处理中,以间作体系接种土著AMF与施用适量有机N的组合明显促进了设施黄瓜生长和N素利用率。

关键词: 土著丛枝菌根真菌, 间作, 黄瓜, 铵态氮, 硝态氮, 酶活性

Abstract:

The unreasonable application and high residue of nitrogen (N) fertilizer in protected agriculture soil are the main factors that lead to excessive nitrate N accumulation in crops and agricultural non-point source pollution. The study of indigenous arbuscular mycorrhizal fungi (AMF) and intercropping system to enhance the utilization of different forms of N by vegetables, combined with the feedback effect of soil enzyme activity, may provide a basis for efficient utilization of N in protected culturing soil and reduction of soil N residue. A pot experiment with different planting ways [cucumber monoculture, and cucumber-soybean intercropping], different AMF treatments [no AMF(NM), and indigenous AMF inoculation] and different forms of N treatments [no N (N0), organic N (glutamine, 120mg/kg, ON120), and inorganic N (ammonium bicarbonate, 120mg/kg, ION120)] was conducted to reveal the effects of different forms of N, indigenous AMF and cucumber-soybean intercropping on soil enzyme activity and N utilization by cucumber under greenhouse conditions. The results showed that compared with NM treatment, inoculation of indigenous AMF increased the shoot and root biomass and N uptake of cucumber to different extent, and decreased the content of NH4 +-N and NO3 --N in rhizosphere soil. Under the same N application and AMF treatment, the cucumber root mycorrhizal colonization percentage under cucumber-soybean intercropping condition was significantly higher than that under cucumber monoculture condition. Intercropped cucumber also significantly increased shoot and root biomass and N uptake, and significantly decreased NH4 +- N content in soil. Under the condition of intercropping and indigenous AMF treatment, urease activity in cucumber rhizosphere soil treated with ON120 and ION120 was 30% and 14% higher than that in the soil with no N treatment, and the protease and nitrate reductase activities appeared the same trend. Our results indicated that cucumber-soybean intercropping combined with inoculation of indigenous AMF and application of appropriate quantity of organic N significantly promoted cucumber growth and N utilization in protected culture.

Key words: indigenous arbuscular mycorrhizal fungi (AMF), intercropping, cucumber, ammonia nitrogen, nitrate nitrogen, enzyme activity