菌物学报 ›› 2019, Vol. 38 ›› Issue (11): 1918-1929.doi: 10.13346/j.mycosystema.190227

• 研究论文 • 上一篇    下一篇

入侵豚草与本地植物竞争对丛枝菌根真菌多样性的影响

孙建茹,陈雪,桑晓玲,蒙彦良,贾月月,张风娟()   

  1. 河北大学生命科学学院 河北 保定 071002
  • 收稿日期:2019-06-26 接受日期:2019-09-12 出版日期:2019-11-22 发布日期:2019-12-14
  • 通讯作者: 张风娟 E-mail:fengjuanzhang@126.com
  • 作者简介:张风娟,河北大学教授,校级教学名师。现兼任中国入侵生态学会理事、河北省植物学会理事、河北省植物病理学会理事等职。长期从事生物入侵和生态安全方向的研究。先后主持国家自然科学基金、国家博士后项目、河北省专项、河北省自然科学基金等项目20余项。在《Soil Biology and Biochemistry》《Weed Science》《Phytochemistry Analysis》等国际学术期刊上发表60余篇论文。主编和参编包括国家面向21世纪课程、普通高等教育国家级规划教材5部、专著5部。
  • 基金资助:
    国家自然科学基金(31972343);河北省自然科学基金(C2019201059)

Effects of the competition between invasive Ambrosia artemisiifolia and the native plants on species diversity of arbuscular mycorrhizal fungi

Jian-Ru SUN,Xue CHEN,Xiao-Ling SANG,Yan-Liang MENG,Yue-Yue JIA,Feng-Juan ZHANG()   

  1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China
  • Received:2019-06-26 Accepted:2019-09-12 Online:2019-11-22 Published:2019-12-14
  • Contact: Feng-Juan ZHANG E-mail:fengjuanzhang@126.com
  • Supported by:
    Supported by the National Natural Science Foundation of China(31972343);and Hebei National Natural Science Foundation(C2019201059)

摘要:

外来植物入侵常引起入侵地丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)多样性的变化,但AMF多样性的变化与入侵域本地植物种类相关性目前尚不清楚。本研究采用野外同质园试验研究豚草与不同本地植物竞争时对AMF多样性的影响。本试验共设入侵豚草单种、本地植物狗尾草Setaria viridis单种、藜 Chenopodium album单种、黄香草木犀Melilotus officinalis单种、豚草和狗尾草混种、豚草和藜混种、豚草和黄香草木犀混种以及裸土(无任何植物生长)对照8个处理。采集根区土样后分离、鉴定AMF,分析植株生物量和AMF物种多样性变化间的关系。结果表明,对豚草而言,单种处理中生物量均高于混种处理;豚草分别与3种本地植物竞争时,与黄香草木犀混种中豚草竞争能力最强,而与藜混种中竞争能力最弱。豚草分别与3种本地植物混种时土壤中AMF种丰度和香农-威纳指数分别显著高于豚草单种和本地植物单种,其中豚草与藜混种中AMF的种丰度和香农-威纳指数最高,与狗尾草混种中AMF的种丰度和香农-威纳指数最低,而豚草分别与3种本地植物混种处理中孢子密度显著低于本地植物单种(豚草与黄香草木犀混种除外)。豚草单种、狗尾草单种、藜单种、豚草分别与狗尾草和藜混种4个处理中AMF优势种均为网状球囊霉Glomus reticulatum和缩隔球囊霉Septoglomus constrictum;黄香草木犀单种中优势种是网状球囊霉、缩隔球囊霉和根内根孢囊霉Rhizophagus Intraradices,豚草与黄香草木犀混种中优势种是网状球囊霉和根内根孢囊霉;狗尾草单种、豚草与狗尾草混种和豚草与藜混种3个处理的特有种分别是木薯根孢囊霉Rhizoglomus manihotis、近明球囊霉Claroideoglomus claroideum和美丽盾巨孢囊霉Scutellospora calospora。狗尾草单种生物量与孢子密度呈显著正相关,豚草与狗尾草混种中豚草的生物量与均匀度呈显著负相关,豚草与黄香草木犀混种中豚草的生物量与孢子密度呈显著正相关,与香农威纳指数呈显著负相关。综上可知,豚草与本地植物竞争对AMF多样性的影响具有异质性,这与本地植物种类密切相关。

关键词: 豚草, 丛枝菌根真菌, 竞争, 多样性

Abstract:

Invasion plants often cause changes in the diversity of arbuscular mycorrhizal fungi (AMF) in invaded areas, while the correlation between the changes of AMF diversity and native plant species in invaded areas is still unclear. Field homogeneity garden experiment was carried out to study the effect of the competition between invasive Ambrosia artemisiifolia and native plants on the diversity of AMF. Eight treatments designed included monoculture of Ambrosia artemisiifolia, monoculture of native Setaria viridis, monoculture of native Chenopodium album, monoculture of native Melilotus officinalis, mixed culture of A. artemisiifolia and Setaria viridis, mixed culture of A. artemisiifolia and Chenopodium album, mixed culture of A. artemisiifolia and Melilotus officinalis, and bare soil (without any plant growth) as experimental control. AMF were sampled from rhizosphere soil and plant biomass and diversity of AMF were investigated. The results showed that the biomass of monocultured A. artemisiifolia plants was higher than that of the plants in mixed culture. The competitive ability of A. artemisiifolia was the strongest in mixed culture of A. artemisiifolia and M. officinalis, but the weakest in mixed culture of A. artemisiifolia and S. viridis. The species richness and Shannon Weiner index of AMF in rhizosphere soil of mixed culture of A. artemisiifoli and respective three native plants were significantly higher than that in monoculture of A. artemisiifolia and three native plants, showing the highest in mixed culture of A. artemisiifoli and Chenopodium album, but the lowest in mixed culture of A. artemisiifoli and S. viridis. The AMF spore density in the rhizosphere soil in mixed culture of A. artemisiifolia and respective tested native plants was significantly lower than that in monoculture of native plants (except for A. artemisiifolia and M. officinalis mixture). Glomus reticulatum and Septoglomus constrictum were dominant in the soil of monocultures of A. artemisiifolia, S. viridis, C. album and the mixed culture of A. artemisiifoli and S. viridis or C. album. Glomus reticulatum, S. constrictum and Rhizophagus intraradices were dominant in the soil of monoculture of M. officinalis. Glomus reticulatum and R. intraradices were dominant in mixed culture of A. artemisiifolia and M. officinalis. Rhizoglomus manihotis, Claroideoglomus claroideum and Scutellospora calospora were dominant in the soil of monoculture of S. viridis, and mixed culture of A. artemisiifolia and S. viridis or C. album. There was a significant positive correlation between plant biomass and AMF spore density in the soil of monoculture of S. viridis, but a significant negative correlation was found between the biomass and uniformity of A. artemisiifolia in mixed culture of A. artemisiifolia and S. viridis. A significant positive correlation was found between the biomass of A. artemisiifolia and the AMF spore density in the soil of mixed culture of A. artemisiifolia and M. officinalis, significantly negative correlation occurred between the plant biomass and the Shannon Weiner index of AMF. In conclusion, the effect of competition between A. artemisiifolia and native plants on the diversity of AMF is heterogeneous, and this phenomenon is closely related to native plant species.

Key words: Ambrosia artemisiifolia, arbuscular mycorrhizal fungi, competition, diversity