Abstract: Transcriptional analysis was performed for seven glycoside hydrolase genes identified by our previously RNA-seq, aiming to identify new genes whose protein products promote the lignocellulose degradation, and provide rational targets for optimizing cellulase system. The structure features of proteins encoded by selected seven genes were predicted via bioinformatic analysis. Quantitative real time PCR was used to analyze the transcriptional levels of seven genes encoding glycoside hydrolases (gene number designated as No. 4–10) along with three major cellulase genes (gene No. 1–3) of cbh1, cbh2 and egl1, under different carbon sources in QM 9414 and RUT C30 strains, respectively. Bioinformatic analysis showed that the protein products of seven genes belong to glycoside hydrolase family 47 (No. 4 and 5), 92 (No. 6–8), 16 (No. 9), and 31 (No. 10), respectively, and contain typical signal peptide sequences. The transcriptional levels of cbh1, cbh2, egl1 were significantly increased under induction of cellulase. Compared to lactose, cellulose induced higher fold change of the three genes in QM 9414 strain on mRNA level. However, in RUT C30 strain, mRNA abundances of the three genes were more abundant when the fungus was grown on lactose than that on cellulose as sole carbon source. The transcriptional levels of seven glycoside hydrolase genes showed the same tendency as those of the three major cellulase genes. The fold changes of gene No. 8 encoding α-mannosidase as well as gene No. 9 encoding β-glucanase were secondary to those of three major cellulase genes under the cellulose induction. However, the transcriptional levels of gene No. 8 and 9 showed more significant alterations when Trichoderma reesei grew on glycerol than that on cellulose. No significant alteration of transcriptional level was observed for gene No. 4, which is probably constitutively expressed by the fungus. The results indicated that the expressions of gene No. 5–10 were significantly dependent on carbon sources and strains. In addition, we found that seven glycoside hydrolase genes and three major cellulase genes were coordinately induced by cellulose and lactose.