贵州地区茯砖茶“金花菌”的分离和分子鉴定

谭玉梅, 王亚萍, 葛永怡, 任秀秀, 王玉臣, 刘作易

Isolation and molecular identification of Aspergillus cristatus in fermented “fuzhuan” brick tea from Guizhou Province

Yu-Mei TAN, Ya-Ping WANG, Yong-Yi GE, Xiu-Xiu REN, Yu-Chen WANG, Zuo-Yi LIU

表1 PCR扩增使用的引物

Table 1 Primers used for PCR amplification

基因 Gene 引物名 Primer 引物序列 Primer sequences (5′-3′) 参考文献 References ITS+LSU ITS1 TCCGTAGGTGAACCTGCGG White et al. 1990; Peterson 2000 D2R GGTCGTTTACGACCATTATG β-tubulin Bt2a GGTAACCAAATCGGTGCTGCTTTC Glass & Donaldson 1995 Bt2b ACCCTCAGTGTAGTGACCCTTGGC CaM CF1L GCTGACTCGTTGACCGAAGAG Peterson et al. 2001 CF4 ATTTTTGCATCATGAGCTGAAC RPB2 5F GAYGAYMGWGATCAYTTYGG Liu et al. 1999 7CR CCCATRGCTTGYTTRCCCAT