基于香草醛-高氯酸显色反应测定灵芝三萜的方法探讨与修正

张倩倩,黄青

Revised method for determining Ganoderma lingzhi terpenoids by UV-Vis spectrophotometry based on colorimetric vanillin perchloric acid reaction

Qian-Qian ZHANG,Qing HUANG

图6 OPUS对吸收峰面积（20 998.58 cm-1-15 002.36 cm-1）积分后,以齐墩果酸的质量为横坐标,积分后吸收峰面积（扣除CK积分）为纵坐标,绘制标准曲线 A：不过原点拟合回归方程;B：过原点拟合回归方程

Fig. 6 Standard curve taking the mass of oleanolic acid as abscissa and post-integration absorption peak area (excluding CK integral) as ordinate after integration of OPUS with absorption peak area (20 998.58cm-1-15 002.36cm-1). A: Not fix intercept at 0; B: Fix intercept at 0.