冠突曲霉 veA基因缺失型与野生型的差异代谢物研究
任春光, 谭玉梅, 任秀秀, 王亚萍, 刘永翔, 刘作易

Differential metabolite analysis of the veA gene deletion and wild type strains of Aspergillus cristatus
Chun-Guang REN, Yu-Mei TAN, Xiu-Xiu REN, Ya-Ping WANG, Yong-Xiang LIU, Zuo-Yi LIU
表1 GC-MS检测冠突曲霉 ∆veA缺失型和野生型孢内代谢物
Table 1 The intracellular metabolites identified by GC-MS of ∆veA mutant and wild type E4 of Aspergillus cristatus in 48h of incubation
物质名称
Name		 保留指数
RI		 质荷比
m/z		 保留时间
RT		 ∆veA-48h vs E4-48h
VIP P-value
Pyruvic acid 1057.3 88.996 365.556 0.992 0.026
Lactic acid+ 1068.7 190.029 375.828 0.637 0.186
Glycolic acid 1082.6 177.003 388.957 0.675 0.159
Alanine+ 1109.7 116.000 414.018 0.159 0.752
3-hydroxypropanoic acid 1150.8 176.994 451.248 0.052 0.918
Monomethylphosphate 1186.9 241.000 484.196 0.254 0.612
Malonic acid+ 1211.2 233.000 505.48 0.911 0.045
2-oxoisocaproic acid 1222 200.066 514.791 1.044 0.017*
Valine+ 1224.3 144.100 517.0325 0.380 0.444
4-hydroxy-butyric acid+ 1241.1 116.973 531.563 1.071 0.013*
Benzoic acid+ 1251.7 178.993 540.413 0.695 0.145
Urea+ 1260.5 189.009 552.317 0.890 0.052
Ethanolamine 1275.2 174.095 560.518 0.637 0.186
Leucine+ 1281.6 158.100 565.855 0.442 0.371
Glycerol+ 1295.8 205.000 578.999 1.228 0.002*
Phosphoric acid 1297.7 299.098 580.558 0.357 0.473
Threonine+ 1307.5 117.000 587.979 1.292 0.001*
Proline+ 1310.4 142.075 590.1685 0.162 0.747
Glycine+ 1319 174.099 597.6325 0.137 0.786
Succinic acid+ 1320.4 247.099 598.405 1.198 0.003*
Glyceric acid 1343.1 292.100 616.84 0.818 0.079
Uracil 1348.1 99.000 621.051 1.249 0.002*
Fumaric acid+ 1351.2 245.000 623.5765 1.399 0.000*
Itaconic acid+ 1359.1 259.024 630.0095 1.297 0.001*
Homoserine 1364.1 146.000 634.253 0.265 0.597
Serine+ 1372.1 204.100 640.5865 0.257 0.608
Mevalonic acid-1,5-lactone 1376.4 145.000 644.4185 1.390 0.000*
2-methyl-1,3-butanediol 1390.9 117.000 655.672 1.138 0.007*
Allothreonine 1399.2 117.000 662.574 0.214 0.670
Glutaric acid+ 1408 186.000 610.2085 1.479 0.000*
N,N-di-(2-hydroxyethyl)-methanamine 1430.6 160.000 686.158 1.143 0.006*
Beta-alanine+ 1436.1 174.010 686.804 0.239 0.633
Erythronic acid-1,4-lactone 1443.3 233.065 696.1045 1.111 0.009*
Nicotinamide+ 1485.9 179.000 729.7865 1.503 0.000*
Malic acid+ 1501.7 233.100 740.0755 0.721 0.129
Erythritol 1519.2 217.100 752.906 1.358 0.000*
Threitol 1530 217.100 759.329 1.407 0.000*
Pyroglutamic acid+ 1533.6 156.000 763.822 0.626 0.195
Aspartic acid+ 1534.4 100.000 764.139 0.676 0.158
N-acetylglutamic acid 1539.8 84.000 767.3565 0.748 0.113
2,4,6-tri-tert-butylbenzenethiol 1552.7 263.186 777.298 1.121 0.008*
Phenylalanine+ 1554.6 120.000 778.5835 1.251 0.002*
Threonic acid+ 1563.7 292.100 785.317 1.281 0.001*
Citramalic acid 1569.9 247.100 789.797 1.343 0.000*
6-hydroxy-3-pyridinecarboxylic acid 1577.8 268.071 795.435 1.112 0.009*
Tetronic acid 1580.1 292.100 797.1945 0.883 0.054
2-hydroxyglutaric acid+ 1586 129.000 801.084 1.346 0.000*
3-hydroxy-3-methylglutaric acid 1614.2 247.088 820.943 0.912 0.045
Glutamic acid+ 1632.2 246.100 832.864 1.000 0.033 *
4-hydroxybenzoic acid 1633.3 267.084 834.121 0.858 0.063
Glucopyranose 1668.9 204.093 862.339 0.660 0.169
2-ketogluconic acid 1675 204.093 862.339 0.660 0.169
Arabinose+ 1687.7 103.000 871.006 0.472 0.338
Ribose+ 1703 103.000 881.274 0.862 0.062
Lysine+ 1718 84.016 890.908 0.701 0.142
Arabinitol+ 1747.4 217.100 910.159 1.545 0.000*
Glycerol-2-phosphate 1749 299.085 911.122 1.379 0.000*
Aconitic acid 1759 229.091 917.662 1.034 0.018*
2-desoxy-pentos-3-ylose dimethoxyamine 1777.3 231.100 929.408 0.823 0.077
Glycerol-3-phosphate 1784.7 299.100 934.438 1.478 0.000*
alpha-d-methylfructofuranoside 1801.5 217.100 944.501 0.132 0.793
Ribonic acid 1809.5 292.100 950.272 1.411 0.000*
3-deoxyglucitol 1813.2 103.000 952.845 0.283 0.571
Hypoxanthine 1818.6 265.100 956.121 1.185 0.004*
Citric acid+ 1844.1 273.100 970.536 0.176 0.726
2-methyl-citric acid 1862 287.095 982.415 1.468 0.000*
O-methyl-inositol 1864 217.100 983.986 1.430 0.000*
Diisobutyl-phthalate 1874.4 149.000 990.058 0.738 0.119
Tyrosine+ 1897.1 179.100 1004.200 1.309 0.001*
Fructose+ 1906.5 103.059 1012.806 1.191 0.004*
Gluconic acid-1,5-lactone 1915.6 220.100 1014.068 1.523 0.000*
Glucose+ 1934.2 319.193 1026.326 1.495 0.000*
Galactose+ 1952.6 319.191 1036.921 1.071 0.013*
Mannitol+ 1967 217.100 1045.580 0.608 0.209
Galactonic acid 1994 292.000 1060.689 1.103 0.010*
Pantothenic acid 2014.2 103.000 1070.657 0.906 0.047
Galactaric acid 2041.1 333.100 1087.941 1.552 0.000*
Hexadecanoic acid 2047.9 117.000 1091.156 0.907 0.047
Glucaric acid 2058.2 292.100 1095.979 1.041 0.017*
9,12-octadecadienoic acid (z,z)-, methyl ester 2094.8 81.005 1118.112 0.184 0.715
9-octadecenoic acid (z)-, methyl ester 2100.3 83.000 1121.075 0.403 0.416
N-acetyl glucosamine 2116.8 205.100 1129.701 1.114 0.009*
Myo-inositol+ 2126.8 305.100 1135.123 0.917 0.044
Uric acid+ 2128.4 441.200 1136.404 1.080 0.012*
Guanine+ 2144.8 352.136 1145.067 0.564 0.247
9,12-(z,z)-octadecadienoic acid+ 2213.3 81.003 1181.329 1.205 0.003*
9-(z)-octadecenoic acid+ 2218.1 117.000 1183.579 1.374 0.000*
Octadecanoic acid+ 2243.4 117.000 1196.418 1.000 0.033*
Fructose-6-phosphate+ 2356.5 299.088 1249.244 0.026 0.959
Sorbitol-6-phosphate 2403.1 299.095 1277.587 1.104 0.010*
Insitol-1-phosphate 2468.7 318.117 1307.646 0.192 0.702
Uridine+ 2477.4 217.100 1313.227 1.344 0.000*
Inosine+ 2603 217.100 1370.676 1.474 0.000*
Adenosine+ 2621.3 245.087 1378.718 0.652 0.175
Guanosine+ 2799 245.100 1454.609 0.223 0.657
Trehalose+ 2809.8 204.100 1460.215 1.494 0.000*
Gentibiose 2889.5 361.200 1489.088 0.631 0.191
Melibiose 2944 204.100 1515.389 0.843 0.069
Galactinol 2996.6 204.100 1539.958 1.041 0.017*