HPLC法测定灵芝孢子粉中三萜含量

杨志空,韩伟,冯娜,张劲松,王晨光,周靖,王金艳,唐庆九

Determination of triterpenoids in Ganoderma lingzhi spore powder by HPLC

Zhi-Kong YANG,Wei HAN,Na FENG,Jing-Song ZHANG,Chen-Guang WANG,Jing ZHOU,Jin-Yan WANG,Qing-Jiu TANG

表1 不同三萜类成分方法学数据

Table 1 Data of methodology of different triterpenes

标准溶液 Standard samples 回归方程 Regressive equations 相关系数 Correlative coefficient 线性范围 Linear bounds (μg/mL) 精密度 Precision (%) 重复性 Repeatability (%) 稳定性 Stability (%) 灵芝酸I Ganoderic acid I Y=1.2e+004X-4.01e+003 0.9999 1.563-100 4.60 3.64 4.39 灵芝烯酸C Ganoderenic acid C Y=1.68e+004X-2.24e+002 1.0000 0.781-50 4.56 3.86 2.98 灵芝酸C2 Ganoderic acid C2 Y=6.79e+003X-7.47e+002 0.9995 1.563-100 4.52 4.96 2.81 灵芝酸C6 Ganoderic acid C6 Y=1.79e+003X-2.54e+003 0.9999 1.563-100 4.96 4.33 1.82 灵芝酸G Ganoderic acid G Y=1.27e+004X-5.22e+003 0.9999 1.563-100 4.75 3.66 3.62 灵芝酸B Ganoderic acid B Y=6.89e+003X-1.93e+003 1.0000 1.563-100 4.79 3.94 3.76 灵芝烯酸A Ganoderenic acid A Y=1.28e+004X-1.36e+003 0.9997 0.781-50 4.61 2.84 1.72 灵芝酸A Ganoderic acid A Y=1.05e+004X-2.13e+003 0.9997 1.563-100 4.64 2.55 4.58 灵芝烯酸E Ganoderenic acid E Y=5.06e+004X-1.76e+003 0.9998 0.781-50 4.65 4.17 2.54 赤芝酸A Lucidenic acid A Y=8.86e+003X-4.56e+003 1.0000 0.781-50 4.62 3.17 4.54 灵芝烯酸D Ganoderenic acid D Y=2.48e+004X-2.03e+003 1.0000 0.781-50 4.66 3.15 4.89 灵芝酸D Ganoderic acid D Y=8.04e+003X-3.87e+003 1.0000 1.563-100 4.69 3.48 3.09 灵芝酸F Ganoderic acid F Y=6.38e+003X-2.76e+003 1.0000 1.563-100 4.86 4.57 3.50