葡萄酒有孢汉逊酵母属分子指纹图谱分析

田进,吴成,杨金仙,王春晓

The molecular fingerprinting analysis of Hanseniaspora in wine

Jin TIAN,Cheng WU,Jin-Xian YANG,Chun-Xiao WANG

表1 有孢汉逊酵母属菌种水平分子鉴定结果

Table 1 The molecular identification results of Hanseniaspora at species level

菌种名称和编号 Species and strain designations 26S rRNA基因D1/D2区域序列比对分析 D1/D2 sequencing analysis of 26S rRNA gene 5.8S-ITS-RFLP分析 5.8S-ITS-RFLP analysis 模式菌株编 号/序列号 Type strain number/GenBank accession number 本土菌株序列号 GenBank accession number of indigenous isolates 序列相似性 Identity percentage PCR产 物大小 Size of PCR products (bp) HaeIII (bp) MboII (bp) DdeI (bp) H. opuntiae 参考菌株：CECT11027 Reference strain: CECT11027 本土菌株：52株 Indigenous strains: 52 CBS8733/AJ512453 MN203644, MN203645 99.82% 780 780 360 340+170 +90+80 H. uvarum 参考菌株：CBS314 Reference strain: CBS314 本土菌株：63株 Indigenous strains: 63 CBS314/U84229 MN203647— MN203650, MK581163 99.82% 780 780 380+280 +120 260+170 +90+80 H. occidentalis 本土菌株：3株 Indigenous strains: 3 CBS2592/U84225 MK581150 99.64% 780 600+120 380+200 +170 550+110 H. vineae 参考菌株：CBS6555 Reference strain: CBS6555 CBS2171/U84224 — — 780 640+90 380+200 +160 440+220 +120 H. guilliermondii 参考菌株：CBS1972 Reference strain: CBS1972 CBS465/U84230 — — 780 780 280+220 +140 340+170 +90+80 H. osmophila 参考菌株：CBS313 Reference strain: CBS313 CBS313/U84228 — — 780 420+140 +90 380+200 +160 670+110