蛋白激酶Cla4对黄曲霉生长发育、毒素合成和致病力的影响

秦岭,李晓雯,李丁,赵家儒,汪世华,袁军

Protein kinase Cla4 regulates morphology development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus

QIN Ling,LI Xiao-Wen,LI Ding,ZHAO Jia-Ru,WANG Shi-Hua,YUAN Jun

表3 本研究过程中所用引物

Table 3 PCR primers used in this study

Primer Sequence (5’-3’) Characteristics cla4-P1 AAAGTGGTTCAATGCCTAC 5’UTR of Aflcla4 cla4-P3 GGGTGAAGAGCATTGTTTGAGGCGGGACAAAGCCATCAGTA pyrG-F GCCTCAAACAATGCTCTTCACCC A. fumigatus pyrG pyrG-R GTCTGAGAGGAGGCACTGATGC cla4-P6 GCATCAGTGCCTCCTCTCAGACAACTGAGACGGGCTTTAC 3’UTR of Aflcla4 cla4-P8 CTCGATGTGATCCACCTAC cla4-P2 CTCGTCCAGGCTCGTTA Fusion PCR cla4-P7 ATCTTCGTCCGCAGGTA cla4-P9 ATTGCTCCTCCAAGACCG ORF validates primers cla4-P10 GCTCCTTTCGTGGCTGAC cla4-qRT-P9 TGCTCTCACAGATGTCATTG cla4-qRT-P10 GTCCGTTGTTCTTCTTCCA cla4 mutant screen pyrG-907-R ATGACGGCGATGTAGGGA pyrG-919-F CGACATCCTCACCGATTTCA RT-PCR for AFB1 biosynthesis aflQ-qRT-F GTCGCATATGCCCCGGTCGG aflQ-qRT-R GGCAACCAGTCGGGTTCCGG aflR-qRT-F AAAGCACCCTGTCTTCCCTAAC RT-PCR for conidial biosynthesis aflR-qRT-R GAAGAGGTGGGTCAGTGTTTGTAG abaA-qRT-F TCTTCGGTTGATGGATGATTTC abaA-qRT-R CCGTTGGGAGGCTGGGT brlA-qRT-F GCCTCCAGCGTCAACCTTC RT-PCR for sclerotial biosynthesis brlA-qRT-R TCTCTTCAAATGCTCTTGCCTC nsdC-qRT-F GCCAGACTTGCCAATCAC nsdC-qRT-R CATCCACCTTGCCCTTTA nsdD-qRT-F GGACTTGCGGGTCGTGCTA nsdD-qRT-R AGAACGCTGGGTCTGGTGC actin-F ACGGTGTCGTCACAAACTGG actin-R GCGTATCGTCGTTACCTCATC