蛋白激酶Cla4对黄曲霉生长发育、毒素合成和致病力的影响
Protein kinase Cla4 regulates morphology development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus
蛋白激酶Cla4对黄曲霉生长发育、毒素合成和致病力的影响 |
| 秦岭,李晓雯,李丁,赵家儒,汪世华,袁军 |
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Protein kinase Cla4 regulates morphology development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus |
| QIN Ling,LI Xiao-Wen,LI Ding,ZHAO Jia-Ru,WANG Shi-Hua,YUAN Jun |
| 图3 黄曲霉Aflcla4缺失株的菌落生长直径及分生孢子相关表型分析 A：37℃条件下WT、ΔAflcla4-2 和ΔAflcla4-3菌株在PDA、GMM、MM和CM培养基培养3d后的菌落形态;B：菌落直径统计;C：分生孢子梗显微观察（200×）;D：不同培养基上,WT和敲除菌株的分生孢子生产量统计;E：WT和敲除菌株的孢子悬浮液;F：abaA和brlA基因的相对表达量. 星号代表WT和其他菌株之间有显著性差异,**P≤0.01 |
| Fig. 3 Roles of Aflcla4 deleted strains in vegetative growth and conidial formation. A: Colony morphology of the wild-type, the single deletion mutants ΔAflcla4-2 and ΔAflcla4-3 on PDA, GMM, MM and CM media at 37°C for 3d; B: Colony diameters of strains; C: Microscopic analysis of conidial structures (200×); D: Indicates the number of conidia produced by WT and deletion strains; E: Different colors of WT and mutant strains’ conidial suspension (108/mL); F: Relative expression level of abaA and brlA genes in strains. Asterisks indicate significant difference between WT and other strains, ** P≤0.01. |
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