蛋白激酶Cla4对黄曲霉生长发育、毒素合成和致病力的影响

秦岭,李晓雯,李丁,赵家儒,汪世华,袁军

Protein kinase Cla4 regulates morphology development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus

QIN Ling,LI Xiao-Wen,LI Ding,ZHAO Jia-Ru,WANG Shi-Hua,YUAN Jun

图7 Aflcla4对黄曲霉菌核形成的影响 A：WKM培养基上菌核形成情况,下部显示平板局部放大图像;B：菌株菌核形成数量统计;C：菌核形成相关基因（nsdC和nsdD）相对表达量统计,所有菌株37℃条件下培养在WKM培养基平板,7d后用75%酒精喷洗平板. 星号代表WT和其他菌株之间有显著性差异,**P≤0.01. ND：未检出

Fig. 7 Effects of Aflcla4 deletion on sclerotial formation. A: Colony morphology of the strains producing sclerotia; magnified image of each plate is showed on the bottom; B: Analysis of sclerotia produced by the wild-type and two mutant strains; C: Relative expression levels of two genes related to sclerotial formation (nsdC and nsdD). All strains were cultured on the WKM medium at 37°C. After incubation for seven days, cultures were washed with 75% ethanol. Asterisks indicate significant differences between the wild-type and other strains (**P≤0.01). ND: No detected.