糙皮侧耳不同生长时期发酵料中微生物和代谢物的变化
刘芹,孔维丽,徐柯,崔筱,宋凯博,张玉亭

The dynamics of microorganisms and metabolites in culture compost during the cultivation of Pleurotus ostreatus
LIU Qin,KONG Wei-Li,XU Ke,CUI Xiao,SONG Kai-Bo,ZHANG Yu-Ting
表1 五组发酵料样本间的差异代谢物
Table 1 Differential metabolites between five groups of the fermentation material samples
样品名称
Sample name 		P值
P value 		出峰时间
Retention
time (min) 		离子模式
Ion mode 		分子式
Formula 		质荷比
Precursor
mass (Da) 		质量误差
Mass
error
胸腺嘧啶Thymine 0.000704 4.7 [M+H]- C5H6N2O2 125.036 1
脱氧肌苷Deoxyinosine 0.001465 4.7 [M+H]- C10H12N4O4 251.079 -0.1
尿嘧啶Uracil 7.01E-08 4 [M+H]+ C4H4N2O2 113.035 2.7
尿苷Uridine 3.45E-07 4.1 [M+H]- C9H12N2O6 243.062 1.3
尿酸Urocanic acid 2.92E-07 2 [M+H]+ C6H6N2O2 139.05 -1
鸟嘌呤Guanine 0.007398 1.6 [M+H]+ C5H5N5O 152.057 0.2
鸟苷Guanosine 2.98E-08 4.7 [M+H]+ C10H13N5O5 284.099 -0.4
次黄嘌呤Hypoxanthine 3.25E-08 2.7 [M+H]+ C5H4N4O 137.046 0.4
胞苷Cytidine 1.95E-07 1.6 [M+H]+ C9H13N3O5 244.093 -1.2
胞苷一磷酸Cytidine monophosphate 1.50E-08 2.1 [M+H]+ C9H14N3O8P 324.059 -1.3
肌苷Inosine 7.14E-09 4.7 [M+H]+ C10H12N4O5 269.088 -1.2
胞嘧啶Cytosine 2.03E-07 1.6 [M+H]+ C4H5N3O 112.051 3.9
脱氧胞苷Deoxycytidine 1.14E-06 2.3 [M+H]+ C9H13N3O4 228.098 -1
脱氧鸟苷Deoxyguanosine 1.56E-09 4.7 [M+H]+ C10H13N5O4 268.104 -0.9
1-甲基腺苷1-methyladenosine 2.33E-06 3.2 [M+H]+ C11H15N5O4 282.12 -0.5
3-甲基腺嘌呤3-methyladenine 3.72E-11 1.6 [M+H]+ C6H7N5 150.077 -2.4
7-甲基鸟嘌呤7-methylguanine 1.74E-07 3 [M+H]+ C6H7N5O 166.072 -1.2
腺苷一磷酸Adenosine monophosphate 2.16E-09 4.3 [M+H]+ C10H14N5O7P 348.07 0.4
腺苷二磷酸ADP 8.96E-08 1.6 [M+H]+ C10H15N5O10P2 428.037 0.4
L-精氨酸L-arginine 4.89E-07 0.9 [M+H]+ C6H14N4O2 175.119 -0.9
L-谷氨酸L-glutamic acid 7.34E-05 1 [M+H]+ C5H9NO4 148.06 0
L-谷氨酰胺L-glutamine 1.77E-08 1 [M+H]+ C5H10N2O3 147.076 -3.6
L-组氨酸L-histidine 2.15E-05 0.9 [M+H]+ C6H9N3O2 156.077 -2.2
L-异亮氨酸L-isoleucine 1.25E-05 3.1 [M+H]+ C6H13NO2 132.102 2
L-亮氨酸L-leucine 4.15E-11 3.4 [M+H]+ C6H13NO2 132.102 1.5
L-赖氨酸L-lysine 2.65E-06 0.8 [M+H]+ C6H14N2O2 147.113 -0.8
L-蛋氨酸L-methionine 2.51E-07 1.9 [M+H]+ C5H11NO2S 150.058 -1.4
L-苯丙氨酸L-phenylalanine 4.17E-05 4.7 [M+H]+ C9H11NO2 166.086 -0.8
L-天冬氨酸L-aspartic acid 4.16E-05 1 [M+H]- C4H7NO4 132.03 3.6
L-脯氨酸L-proline 3.12E-07 1.1 [M+H]+ C5H9NO2 116.071 1.6
L-苏氨酸L-threonine 2.97E-08 1 [M+H]+ C4H9NO3 120.066 0.6
L-色氨酸L-tryptophan 3.97E-06 4.7 [M+H]+ C11H12N2O2 205.097 -1.2
L-酪氨酸L-tyrosine 6.50E-07 3.4 [M+H]+ C9H11NO3 182.081 -0.4
L-缬氨酸L-valine 3.88E-07 1.5 [M+H]+ C5H11NO2 118.086 -0.2
瓜氨酸Citrulline 3.99E-08 1 [M+H]+ C6H13N3O3 176.103 -2
焦谷氨酸Pyroglutamic acid 8.32E-08 2.7 [M+H]+ C5H7NO3 130.05 0.6
酮亮氨酸Ketoleucine 1.50E-10 5.2 [M+H]- C6H10O3 129.056 4.5
N-乙酰谷氨酸N-acetylglutamic acid 9.17E-06 3.2 [M+H]+ C7H11NO5 190.071 -2.2
N-乙酰基-L-丙氨酸N-acetyl-L-alanine 2.20E-07 3.7 [M+H]- C5H9NO3 130.051 4.7
甘氨酰脯氨酸Glycylproline 5.73E-06 1.6 [M+H]+ C7H12N2O3 173.092 -1.2
精氨琥珀酸Argininosuccinic acid 4.27E-09 1 [M+H]+ C10H18N4O6 291.13 -0.6
鞘氨醇Sphinganine 3.29E-05 8.1 [M+H]+ C18H39NO2 302.305 -0.1
L-肉碱L-carnitine 7.37E-10 1 [M+H]+ C7H15NO3 162.112 -1.5
甘油磷酰胆碱Glycerophosphocholine 2.06E-14 1 [M+H]+ C8H20NO6P 258.11 0.1
甘油酸Glyceric acid 1.51E-08 1.2 [M+H]- C3H6O4 105.019 4.2
甘油3-磷酸Glycerol 3-phosphate 4.06E-10 1 [M+H]- C3H9O6P 171.006 -0.5
烟酰胺单核苷酸
Nicotinamide mononucleotide 		2.97E-08 1.4 [M+H]+ C11H15N2O8P 335.064 0.1
烟酸Nicotinic acid 0.000287 2 [M+H]+ C6H5NO2 124.039 0.9
6-羟基烟酸6-hydroxynicotinic acid 0.000309 2.6 [M+H]- C6H5NO3 138.02 3.2
吡哆醇Pyridoxine 6.67E-07 2.8 [M+H]+ C8H11NO3 170.081 -1.7
D-木糖D-xylose 4.21E-11 1.6 [M+H]- C5H10O5 149.046 3.1
半乳糖醇Galactitol 3.63E-13 1 [M+H]- C6H14O6 181.072 0.6
甘露醇Mannitol 3.63E-13 1 [M+H]- C6H14O6 181.072 0.8
山梨糖醇Sorbitol 3.63E-13 1 [M+H]- C6H14O6 181.072 0.6
N-乙酰-D-氨基葡萄糖
N-acetyl-D-glucosamine 		7.35E-10 1.1 [M+H]+ C8H15NO6 222.097 -1.1
尿苷二磷酸葡萄糖
Uridine diphosphate glucose 		1.29E-07 1.3 [M+H]- C15H24N2O17P2 565.048 1.4
2-异丙基苹果酸2-isopropylmalic acid 1.30E-07 4.8 [M+H]- C7H12O5 175.061 1.8
3-甲基己二酸3-methyladipic acid 3.28E-10 4.8 [M+H]- C7H12O4 159.066 2.7
4-羟基苯甲酸4-hydroxybenzoic acid 9.91E-05 4.8 [M+H]- C7H6O3 137.024 4.6
4-丙酮酸4-pyridoxic acid 4.00E-06 4.6 [M+H]- C8H9NO4 182.046 -0.6
5-氨基戊酸5-aminopentanoic acid 6.64E-05 1.6 [M+H]- C5H11NO2 116.072 4.5
壬二酸Azelaic acid 5.69E-05 5.2 [M+H]- C9H16O4 187.098 1.8
柠苹酸Citramalic acid 9.69E-06 3.4 [M+H]- C5H8O5 147.03 1.3
柠檬酸Citric acid 1.74E-05 2.5 [M+H]- C6H8O7 191.02 2
葡萄糖酸Glucaric acid 4.08E-08 1 [M+H]- C6H10O8 209.03 1
D-核糖5-磷酸D-ribose 5-phosphate 3.35E-06 1.1 [M+H]- C5H11O8P 229.012 0.1
果糖6-磷酸Fructose 6-phosphate 4.71E-09 1 [M+H]- C6H13O9P 259.022 0.5
葡萄糖1-磷酸Glucose 1-phosphate 4.71E-09 1 [M+H]- C6H13O9P 259.022 0.5
葡萄糖6-磷酸Glucose 6-phosphate 4.71E-09 1 [M+H]- C6H13O9P 259.022 0.5
甘露糖6-磷酸Mannose 6-phosphate 4.71E-09 1 [M+H]- C6H13O9P 259.022 0.5
木酮糖5-磷酸Xylulose 5-phosphate 9.74E-07 1.1 [M+H]- C5H11O8P 229.012 0.1
十六烷基二酸Hexadecanedioic acid 1.12E-07 9.5 [M+H]- C16H30O4 285.207 1.9
咪唑乙酸Imidazoleacetic acid 1.51E-06 1.2 [M+H]- C5H6N2O2 125.036 4.2
4-胍基丁酸4-guanidinobutanoic acid 1.02E-11 1.6 [M+H]+ C5H11N3O2 146.092 -0.8
2-氨基异丁酸2-aminoisobutyric acid 1.45E-05 1 [M+H]+ C4H9NO2 104.071 4
N-甲基-α-氨基异丁酸
N-methyl-a-aminoisobutyric acid 		1.30E-08 1 [M+H]+ C5H11NO2 118.086 1.6
异柠檬酸Isocitric acid 0.000147 1.3 [M+H]- C6H8O7 191.02 1
反式阿魏酸trans-ferulic acid 1.63E-06 5.4 [M+H]- C10H10O4 193.051 1.8
异阿魏酸Isoferulic acid 1.63E-06 5.4 [M+H]- C10H10O4 193.051 1.8
L-苹果酸L-malic acid 1.28E-09 1.5 [M+H]- C4H6O5 133.014 3.3
顺丁烯二酸Maleic acid 1.99E-07 2.8 [M+H]- C4H4O4 115.004 3.9
中康酸Mesaconic acid 1.73E-09 2.4 [M+H]- C5H6O4 129.019 2.9
甲基戊二酸Methylglutaric acid 2.53E-07 4.8 [M+H]- C6H10O4 145.051 4.1
油酸Oleic acid 0.000995 15 [M+H]- C18H34O2 281.249 1.1
酮戊二酸Oxoglutaric acid 4.39E-09 1.6 [M+H]- C5H6O5 145.014 2.2
苯乙酮酸Phenylglyoxylic acid 3.06E-09 4.8 [M+H]- C8H6O3 149.024 3.2
苯乳酸Phenyllactic acid 0.000348 5.1 [M+H]- C9H10O3 165.056 1.6
邻苯二甲酸Phthalic acid 2.42E-09 4.9 [M+H]- C8H6O4 165.019 2.2
喹啉酸Quinolinic acid 0.000675 4.6 [M+H]- C7H5NO4 166.015 3.5
癸二酸Sebacic acid 0.000566 5.5 [M+H]- C10H18O4 201.113 0.9
辛二酸Suberic acid 5.73E-06 4.9 [M+H]- C8H14O4 173.082 0.4
琥珀酸Succinic acid 4.11E-08 3.2 [M+H]- C4H6O4 117.019 4.7
创伤酸Traumatic acid 0.000129 6.3 [M+H]- C12H20O4 227.129 1
十一碳二酸Undecanedioic acid 1.67E-05 6 [M+H]- C11H20O4 215.129 1
4-羟基-3-甲基苯甲酸
4-hydroxy-3-methylbenzoic acid 		3.45E-11 5.5 [M+H]+ C8H8O3 153.055 -1
2-氨基苯甲酸2-aminobenzoic acid 3.46E-07 4.7 [M+H]+ C7H7NO2 138.055 -1.9
吲哚-3-羧酸Indole-3-carboxylic acid 0.000249 5.3 [M+H]- C9H7NO2 160.04 3.6
吲哚丙烯酸Indoleacrylic acid 5.90E-12 4.7 [M+H]+ C11H9NO2 188.071 -0.9
吲哚Indole 9.17E-08 5.4 [M+H]+ C8H7N 118.065 0
葫芦巴碱Trigonelline 1.04E-08 1.1 [M+H]+ C7H7NO2 138.055 -0.9