带有报告基因的板栗疫病菌寄生隐赤壳分泌表达突变体库的构建及分泌缺陷突变体的筛选
李茹1,仇金凤1,杨彦彦1,王金子1,3,陈凤月1,陈保善1,2,*(
)
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Construction and screening of the Cryphonectria parasitica mutant library constructed with a reporter gene for secretory pathway
LI Ru1,QIU Jin-Feng1,YANG Yan-Yan1,WANG Jin-Zi1,3,CHEN Feng-Yue1,CHEN Bao-Shan1,2,*()
)
图5. 突变体的表型观察及GUS活性检测
A:部分突变体的菌落表型;B:X-Gluc法检测突变株GUS活性;C:MUG法检测液体培养条件突变株的GUS活性（3次生物学重复）;D:MUG法检测固体培养条件突变株的GUS活性（3次生物学重复）
Fig. 5. Phenotypes and GUS activity identification of mutants.
A: Phenotypes of some mutants; B: Identification of GUS activity in mutants using X-Gluc method; C: GUS activity in liquid cultured mutants using MUG method (three biological replicates); D: GUS activity in solid cultured mutants using MUG method (three biological replicates).
