冬虫夏草菌实时荧光定量PCR内参基因的筛选

苏强军,夏樱霞,谢放,Uwitugabiye VESTINE,陈照禾,周刚

Screening of the reference genes for qRT-PCR analysis of gene expression in Ophiocordyceps sinensis

SU Qiang-Jun,XIA Ying-Xia,XIE Fang,Uwitugabiye VESTINE,CHEN Zhao-He,ZHOU Gang

表1 候选内参基因的引物序列

Table 1 Primer sequences of candidate internal reference genes

基因名称 Gene name 正向引物 Forward primer (5’-3’) 反向引物 Reverse primer (5’-3’) 扩增产物 Amplicon size (bp) Tm (°C) 18S rRNA GCAGTGGCATCTCTCAGTC TCATCGATGCCAGAACC 128 60 APRTase ATGCTGAGCTGTTTAGCG TGCCCTGTTCGTCGTAGA 90 60 β-TUB TACGCCTCTTCGACGATAG GCCGTTGTAGACACCATT 148 60 RPL2 ACCTACCGTCTCCATCAT GTGAACCTGCTGGACAAT 109 60 EF1-α CAAGGGCTCTTTCAAGTATGC GTGACATAGTACCTGGGAGT 114 60 PGI TTCGACCAGTATCTTCATCGC GTGTACTTGACCGACGATCC 83 60 PGM AAGCCCTTTCAGGACCAA GAACGACTCGGTGTAGTG 87 60 H+-ATPase CGCTTCGCGGAAATCTATAC AGACGTTCTTGTTGACGG 90 60 ACT1 CAATCGGCACAACTGGACA GACGACCTGAGCGGAATA 95 60 UBQ CGACATCGAGTTGGACTAC ATACCTGCAATCTGTCCG 82 60 GAPDH GAGGCCGAGAGCCAACTA TTCATCACGACAGCACCA 99 60