冬虫夏草菌实时荧光定量PCR内参基因的筛选
|
|
苏强军,夏樱霞,谢放,Uwitugabiye VESTINE,陈照禾,周刚
|
Screening of the reference genes for qRT-PCR analysis of gene expression in Ophiocordyceps sinensis
|
|
SU Qiang-Jun,XIA Ying-Xia,XIE Fang,Uwitugabiye VESTINE,CHEN Zhao-He,ZHOU Gang
|
|
表2 RNA质检结果
|
Table 2 Results of RNA quality control
|
|
样本
Sample | 浓度
Concentration (μg/μL) | A260/280 | A260/230 | 体积
Volume (μL) | 总量
Total (μg) | 28S/18S | RIN | | A1 | 0.3864 | 2.18 | 1.84 | 20 | 7.73 | 1.7 | 9.5 | | A2 | 0.1979 | 2.19 | 2.28 | 20 | 3.96 | 0.9 | 7.7 | | A3 | 0.1701 | 2.14 | 1.78 | 20 | 3.40 | 1.0 | 7.2 | | B1 | 0.3490 | 2.18 | 1.81 | 20 | 6.98 | 1.7 | 10.0 | | B2 | 0.2323 | 2.17 | 1.77 | 20 | 4.65 | 2.1 | 9.2 | | B3 | 0.1352 | 2.16 | 1.75 | 15 | 2.03 | 1.2 | 7.1 | | C1 | 0.3873 | 2.16 | 2.24 | 20 | 7.75 | 1.5 | 8.8 | | C2 | 0.5354 | 2.23 | 2.00 | 20 | 10.71 | 1.1 | 7.9 | | C3 | 0.2250 | 2.16 | 2.10 | 20 | 4.50 | 1.3 | 7.9 |
|
|
|