冬虫夏草菌实时荧光定量PCR内参基因的筛选

苏强军,夏樱霞,谢放,Uwitugabiye VESTINE,陈照禾,周刚

Screening of the reference genes for qRT-PCR analysis of gene expression in Ophiocordyceps sinensis

SU Qiang-Jun,XIA Ying-Xia,XIE Fang,Uwitugabiye VESTINE,CHEN Zhao-He,ZHOU Gang

表4 BestKeeper程序分析候选内参基因的稳定性表达

Table 4 BestKeeper program analyzes the stability expression of candidate reference genes

基因名称 Gene name 变异系数 CV 标准差 SD APRTase 1.23 0.33 ACT1 1.68 0.43 H+-ATPase 1.8 0.53 UBQ 2.17 0.65 PGM 2.97 0.69 18S rRNA 4.31 0.7 GAPDH 2.37 0.7 EF-1A 3.9 0.73 PGI 3.65 0.86 β-TUB 3.9 0.88 RPL2 3.86 0.9