冬虫夏草菌实时荧光定量PCR内参基因的筛选
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苏强军,夏樱霞,谢放,Uwitugabiye VESTINE,陈照禾,周刚
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Screening of the reference genes for qRT-PCR analysis of gene expression in Ophiocordyceps sinensis
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SU Qiang-Jun,XIA Ying-Xia,XIE Fang,Uwitugabiye VESTINE,CHEN Zhao-He,ZHOU Gang
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表5 RefFinder综合排名结果
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Table 5 Comprehensive ranking results of RefFinder
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基因名称
Gene name | Delta CT排名
Delta CT ranking | geNorm排名
geNorm ranking | NormFinder排名
NormFinder ranking | BestKeeper排名
BestKeeper ranking | 几何均值
Geomean | 综合排名
Comprehensive
ranking | | UBQ | 2 | 2 | 1 | 4 | 1.78 | 1 | | PGM | 1 | 1 | 2 | 5 | 1.86 | 2 | | ACT1 | 4 | 8 | 3 | 2 | 4.12 | 3 | | EF-1α | 3 | 3 | 5 | 8 | 4.49 | 4 | | H+-ATPase | 5 | 9 | 4 | 3 | 5.18 | 5 | | APRTase | 8 | 10 | 7 | 1 | 5.79 | 6 | | 18S rRNA | 6 | 7 | 6 | 6 | 6.48 | 7 | | PGI | 7 | 6 | 8 | 9 | 7.16 | 8 | | β-TUB | 9 | 5 | 9 | 10 | 8.17 | 9 | | RPL2 | 10 | 4 | 10 | 11 | 8.32 | 10 | | GAPDH | 11 | 11 | 11 | 7 | 10.84 | 11 |
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