三种分离自冬虫夏草真菌及培养条件

孟云,唐汉尧,施金铎,王敦

Three fungal strains isolated from stroma of Ophiocordyceps sinensis and their culture conditions

MENG Yun,TANG Han-Yao,SHI Jin-Duo,WANG Dun

表1 本研究所用引物

Table 1 Primers used in this study for molecular identification

基因名称 Gene 引物名称 Primer 序列 Sequence (5’-3’) 参考文献 Reference nrSSU nrSSU-CoF TCTCAAAGATTAAGCCATGC Guhr & Weig 2020 nrSSU-CoR TCACCAACGGAGACCTTG nrLSU LR5 ATCCTGAGGGAAACTTC Vilgalys & Sun 1994 LR0R GTACCCGCTGAACTTAAGC TEF EF1α-EF GCYCCYGGHCAYCGTGAYTTYAT Bischoff et al. 2009 EF1α-ER ATGACACCRACRGCRACRGTYTG Sung et al. 2007 RPB1 RPB1Cr CCNGCDATNTCRTTRTCCATRTA Castlebury et al. 2004 CRPB1A CAYCCWGGYTTYATCAAGAA RPB2 fRPB2-7cR CCCATRGCTTGTYYRCCCAT Liu et al. 1999 fRPB2-5F GAYGAYMGWGATCAYTTYGG ITS ITS1 TCCGTAGGTGAACCTGCGG White et al. 1990 ITS2 GCTGCGTTCTTCATCGGATGC