三种分离自冬虫夏草真菌及培养条件

孟云,唐汉尧,施金铎,王敦

Three fungal strains isolated from stroma of Ophiocordyceps sinensis and their culture conditions

MENG Yun,TANG Han-Yao,SHI Jin-Duo,WANG Dun

表5 不同菌株在不同培养基上生长状况与测量结果

Table 5 Growth characters and measurement result of three isolates grown on different media

菌株 Strain 培养基名称 Medium type 菌丝日生长速率 Mycelial growth rate (mm/d) 菌落形态 Colonial morphology 菌丝长势 Mycelial growth vigor Samsoniella hepiali QH 2019 PDA 1.77±0.12 b 菌落正面为白色;背面的中间为浅橘黄色,边缘为白色 The obverse side of colony was white; the middle of colony reverse side was light orange with a white edge ++ 1/4 SDAY 1.94±0.55 a 菌落正面中间为鸭黄色,边缘为白色;背面中间为黄色, 边缘为白色 The middle of the colony obverse side was yellow with a white edge; the reverse side was yellow with a white edge +++ 查氏培养基 Czapek-Dox medium 1.63±0.13 b 菌落正面为鸭黄色,上有一层白色的气生菌丝, 背面为橘黄色,颜色较深 The obverse side of colony was yellow with a layer of white aerial mycelium and the reverse side was dark orange + Isaria farinosa GL 2019 PDA 2.37±0.20 a 菌落正面中间为鸭黄色且有孢梗束,边缘为白色; 背面为乳白色 The middle of colony obverse side was yellow with coremium, and its edge was white; the reverse side was milk white ++ 1/4 SDAY 2.04±0.40 b 菌落正面为白色,有少量孢梗束;背面为黄色 The obverse side of colony was white with coremium and reverse side was yellow +++ 查氏培养基 Czapek-Dox medium 1.92±0.18 b 菌落正面为白色、边缘为肉色;背面为橘黄色 The obverse side of colony was white and the edge was flesh color; the reverse side was orange + Isaria fumosorosea YN 2019 PDA 2.27±0.27 a 菌落正面中间为肉红色,边缘为白色,孢梗束多; 背面为浅橘黄色,边缘为白色 The middle of colony obverse side was flesh color and its edge was white with thick coremium; the reverse side was light orange with a white edge ++ 1/4 SDAY 1.89±0.16 b 菌落正面为白色,中间有浅肉红色的孢梗束, 上面有少量外渗物;背面为黄色,边缘为白色 The obverse side of colony was white, and its middle was a light flesh color coremium covered with light exosmosis; the reverse side was yellow with a white edge +++ 查氏培养基 Czapek-Dox medium 1.78±0.40 b 菌落正面为白色,无孢梗束;背面为橘黄色,边缘为白色 The obverse side of the colony was white without coremium; the reverse side was orange with a white edge +