葡萄座腔菌Mcm基因家族全基因组鉴定及其对硫化物的响应特性

付静,孙梦,刘俊萍,黄永红

Genome-wide identification and the responses to sulfides of the Mcm gene family in Botryosphaeria dothidea

FU Jing,SUN Meng,LIU Jun-Ping,HUANG Yong-Hong

表1 葡萄座腔菌Mcm基因家族qRT-PCR引物

Table 1 The qRT-PCR primers for Mcm gene family of Botryosphaeria dothidea

基因 Gene 上游引物 Forward primer sequence (5′-3′) 下游引物 Reverse primer sequence (5′-3′) BdMcm2 GTAAAGTCGCTCAGGGCAAG AGCGTCCACTCGCTAGTCAT BdMcm3 GAAGACGAGAACGGAAGTGC GTCAAATTTGCGTGGTTGTG BdMcm4 GCAGCAAGTACAACCCCAAT GAGGTACATGCCGACCAGAT BdMcm5 CGCAACGAGAATGAGGACAG TGTGCCAGCTCCTCATTGTA BdMcm6 CTAACCAGACTGGCGAGGAG TTGATGACCTGGTGTCCGTA BdMcm7 AAAGCTGGAATCACCACCAC GGTTTCAGGGTGCTTGTTGT Actin GTTCAGACCGCCCTTTGCT AGCCTTGCGACGGAACATA