油酸促进灵芝三萜液态深层发酵的工艺研究及规模化验证

苏晓薇,唐庆九,张劲松,冯娜,王金艳,周帅,冯杰,俞苓

Synthesis of triterpenes in liquid submerged fermentation of Ganoderma lingzhi promoted by oleic acid

SU Xiao-Wei,TANG Qing-Jiu,ZHANG Jing-Song,FENG Na,WANG Jin-Yan,ZHOU Shuai,FENG Jie,YU Ling

表2 添加高温灭菌油酸和过滤除菌两种方式下的响应面实验方案及结果

Table 2 Design and results of adding oleic acid moist heat sterilized and filtered sterilized

试验点 Run No. 时间 Time (h) 浓度 Concentration (%, V/V) 高温灭菌油酸条件下三萜含量 Triterpene content under the condition of adding moist heat sterilized oleic acid (mg/g) 过滤除菌油酸条件下三萜含量 Triterpene content under the condition of adding filtered sterilized oleic acid (mg/g) 1 0 1 33.35±0.88 40.41±1.75 2 7 1.35 40.91±1.38 45.74±0.09 3 7 0.65 26.21±2.28 31.64±1.16 4 24 1 42.28±1.50 40.35±1.50 5 24 0.5 31.25±1.31 35.31±0.69 6 24 1 41.49±1.35 43.36±0.75 7 24 1.5 43.70±1.19 47.21±1.37 8 24 1 47.80±0.50 42.08±1.85 9 41 1.35 45.53±0.47 44.59±0.34 10 41 0.65 42.23±1.63 37.12±0.22 11 48 1 36.56±1.79 43.39±1.09