油酸促进灵芝三萜液态深层发酵的工艺研究及规模化验证

苏晓薇,唐庆九,张劲松,冯娜,王金艳,周帅,冯杰,俞苓

Synthesis of triterpenes in liquid submerged fermentation of Ganoderma lingzhi promoted by oleic acid

SU Xiao-Wei,TANG Qing-Jiu,ZHANG Jing-Song,FENG Na,WANG Jin-Yan,ZHOU Shuai,FENG Jie,YU Ling

表6 放大规模的响应面验证

Table 6 Response surface validation at large scales of fermentation

规模 Scale 类别 Category 高温灭菌 Moist heat sterilization 过滤除菌 Filtration sterilization 三萜含量 Triterpene content (mg/g) 菌丝体干重 Mycelial dry weight (g/L) 三萜含量 Triterpene content (mg/g) 菌丝体干重 Mycelial dry weight (g/L) 1 000mL摇瓶 1 000mL shaking flask 对照组 Control 16.62±0.22 7.22±0.12 16.62±0.22 7.22±0.12 响应面优化组 Optimal response surface group 32.18±0.89 13.63±0.14 32.43±0.17 14.01±0.01 响应面中心组 Response surface center group 29.84±0.85 11.64±0.11 32.35±0.87 12.78±0.06 3L发酵罐 3L fermenter 对照组 Control 17.67±0.29 7.76±0.15 17.67±0.29 7.76±0.15 响应面优化组 Optimal response surface group 28.66±0.44 11.67±0.29 25.13±0.47 13.20±0.04