油酸促进灵芝三萜液态深层发酵的工艺研究及规模化验证

苏晓薇,唐庆九,张劲松,冯娜,王金艳,周帅,冯杰,俞苓

Synthesis of triterpenes in liquid submerged fermentation of Ganoderma lingzhi promoted by oleic acid

SU Xiao-Wei,TANG Qing-Jiu,ZHANG Jing-Song,FENG Na,WANG Jin-Yan,ZHOU Shuai,FENG Jie,YU Ling

表7 不同发酵规模下的灵芝三萜HPLC图的峰面积

Table 7 Peak area of fermentation parameters in different fermentation scales

规模 Scale 250mL摇瓶 250mL shaking flask 1 000mL摇瓶 1 000mL shaking flask 3L发酵罐 3L fermenter 名称 Name 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 主要出峰数 The number of main peaks 4 6 6 4 5 5 6 7 8 总面积 Total area (mAU·min) 40 841 184 009 223 336 83 725 687 430 236 126 352 088 545 365 792 093