油酸促进灵芝三萜液态深层发酵的工艺研究及规模化验证

苏晓薇,唐庆九,张劲松,冯娜,王金艳,周帅,冯杰,俞苓

Synthesis of triterpenes in liquid submerged fermentation of Ganoderma lingzhi promoted by oleic acid

SU Xiao-Wei,TANG Qing-Jiu,ZHANG Jing-Song,FENG Na,WANG Jin-Yan,ZHOU Shuai,FENG Jie,YU Ling

表8 不同培养规模下的灵芝菌丝体发酵参数比较

Table 8 Fermentation parameters in different fermentation scales

参数名称 Parameters 发酵规模 Fermentation scales 250mL摇瓶 250mL shaking flask 1 000mL摇瓶 1 000mL shaking flask 3L发酵罐 3L fermenter 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 对照 Control 高温灭菌 Moist heat sterilized oleic acid 过滤除菌 Filtered sterilized oleic acid 初始还原糖浓度 Initial reducing sugar concentration (g/L) 20 20 20 20 20 20 20 20 20 最终还原糖浓度 Final reducing sugar concentration (g/L) 5.4 11.2 11.5 3.2 12.25 13.65 15 15.4 15.5 发酵时间 Fermentation time (h) 168 168 168 168 168 168 168 168 168 最大菌丝体生物量 Maximum mycelial biomass (g/L) 10.12 14.42 20.79 7.22 13.63 14.01 7.76 11.67 13.20 灵芝三萜得率 Triterpene yield (g/L) 0.212 0.610 0.886 0.120 0.463 0.461 0.137 0.334 0.332 还原糖消耗速率 Reducing sugar consumption rate [g/(L·h)] 0.087 0.052 0.051 0.100 0.046 0.038 0.030 0.027 0.027 菌丝体对还原糖得率 Mycelial yield on reducing sugar (g/g) 0.693 1.639 2.446 0.430 1.759 2.206 1.552 2.537 2.933 灵芝三萜对还原糖得率 Triterpene yield on reducing sugar (×10-2g/g) 1.449 6.933 10.421 0.715 5.971 7.254 2.740 7.261 7.378 菌丝体生产强度 Mycelial productivity [g/(L·h)] 0.060 0.086 0.124 0.043 0.081 0.083 0.046 0.069 0.079 灵芝三萜生产强度 Triterpene productivity [10-3g/(L·h)] 1.259 3.632 5.273 0.715 2.754 2.742 0.816 1.991 1.975