基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究

彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟

Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method

PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei

表2 三萜标准品的线性回归方程及检测限、定量限

Table 2 Linear regression data, LOD and LOQ of the triterpenoid standard

三萜标准品 Triterpenoid standard 回归方程 Regressive equations R2 检测限 LOD (ng) 定量限 LOQ (ng) Ganoderic acid I Y=8.735×102X-8.725×103 0.9969 1.52 4.37 Ganoderenic acid C Y=7.865×10X-6.482×102 0.9965 1.14 3.76 Ganoderic acid C2 Y=1.518×102X-1.831×103 0.9968 2.11 6.31 Ganoderic acid C6 Y=3.359×103X-3.753×103 0.9975 1.46 4.38 Ganoderic acid G Y=3.417×102X-3.997×103 0.9974 1.04 3.24 Ganoderenic acid B Y=1.201×103X-1.742×104 0.9975 1.77 5.46 Ganoderic acid B Y=1.098×103X-1.521×104 0.9977 1.04 5.39 Ganoderenic acid A Y=1.175×102X-9.416×104 0.9967 1.27 4.86 Ganoderic acid K Y=6.971×102X-8.832×103 0.9971 2.01 3.94 Ganoderic acid A Y=4.668×102X-5.997×103 0.9969 2.16 5.36 Ganoderic acid H Y=9.463×102X-1.157×104 0.9968 1.52 4.95 Lucidenic acid A Y=3.006×102X-2.821×103 0.9968 1.09 6.06 Ganoderic acid N Y=5.227×102X-6.549×103 0.9970 1.27 4.65 Ganoderenic acid H Y=3.953×103X-5.466×104 0.9966 1.31 4.97 Ganoderenic acid D Y=3.466×103X-4.006×104 0.9967 1.05 5.28 Ganoderic acid D Y=8.780×102X-9.416×103 0.9966 1.12 6.05 Ganoderic acid F Y=1.551×103X-2.154×104 0.9966 1.03 4.93 Ganoderic acid DM Y=2.084×10X-1.095×102 0.9957 1.37 5.96 O-acetyl-16α-hydroxytra- metenolic acid Y=2.801×10X-3.339×102 0.9956 1.24 5.27