基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究
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彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟
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Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method
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PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei
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表4 响应面实验设计及结果
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Table 4 Design and results for response surface method
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| Run | Factor | Response | A: Ethanol
concentration (%) | B: Liquid-to-material
ratio (mL/g) | C: Extraction
time (h) | D: Triterpenoid
content (mg/g) | E: Extract
yield (%) | | 1 | -1 | 0 | -1 | 7.6126 | 2.73 | | 2 | 0 | -1 | -1 | 7.5185 | 2.46 | | 3 | 0 | 1 | -1 | 8.6299 | 2.99 | | 4 | 0 | 0 | 0 | 8.5524 | 2.81 | | 5 | 0 | -1 | 1 | 7.9230 | 2.56 | | 6 | -1 | 0 | 1 | 8.2636 | 2.91 | | 7 | 1 | 0 | -1 | 7.4777 | 2.57 | | 8 | 0 | 1 | 1 | 9.0285 | 3.06 | | 9 | -1 | 1 | 0 | 8.6749 | 3.03 | | 10 | 0 | 0 | 0 | 8.5795 | 2.84 | | 11 | 0 | 0 | 0 | 8.5660 | 2.79 | | 12 | 1 | -1 | 0 | 7.1845 | 2.36 | | 13 | 1 | 1 | 0 | 7.9888 | 2.76 | | 14 | 1 | 0 | 1 | 7.6104 | 2.63 | | 15 | -1 | -1 | 0 | 7.3054 | 2.47 |
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