基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究

彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟

Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method

PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei

表5 以三萜含量为响应值的响应面回归模型的方差分析

Table 5 Variance analysis of response surface regression model with triterpenoid content as response value

Source df Mean square F-value P-value Significance Model 9 0.5383 3894.2208 <0.001 Very significant A: Ethanol concentration 1 0.3180 2300.9773 <0.001 Very significant B: Liquid-to-material ratio 1 2.4098 17434.2807 <0.001 Very significant C: Extraction time 1 0.3147 2277.0936 <0.001 Very significant AB 1 0.0799 577.7910 <0.001 Very significant AC 1 6.72E-02 485.8799 <0.001 Very significant BC 1 8.70E-06 0.0630 0.8119 Not significant A² 1 1.5876 11486.2668 <0.001 Very significant B² 1 0.0548 396.5123 <0.001 Very significant C² 1 0.1056 764.3833 <0.001 Very significant Residual 5 0.0001 Lack of fit 3 0.0001 0.5880 0.6791 Not significant Pure error 2 0.0002 Cor total 14 R²=0.9987 R²adj=0.9996