基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究

彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟

Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method

PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei

表6 以提取物得率为响应值的响应面回归模型的方差分析

Table 6 Variance analysis of response surface regression model with extract yield as response value

Source df Mean square F-value P-value Significance Model 9 0.0734 124.8029 <0.001 Very significant A: Ethanol concentration 1 0.0841 142.8612 <0.001 Very significant B: Liquid-to-material ratio 1 0.4950 841.3810 <0.001 Very significant C: Extraction time 1 0.0210 35.7153 0.0019 Very significant AB 1 0.0064 10.8782 0.0215 Significant AC 1 0.0036 6.1190 0.0563 Not significant BC 1 0.0002 0.3824 0.5634 Not significant A² 1 0.0430 73.0889 0.0004 Very significant B² 1 0.0094 15.9523 0.0104 Significant C² 1 7.76E-05 0.1318 0.7314 Not significant Residual 5 0.0006 Lack of fit 3 0.0006 0.8816 0.5703 Not significant Pure error 2 0.0006 Cor total 14 R²=0.9553 R²adj=0.9876