基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究

彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟

Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method

PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei

图1 灵芝三萜标准品超高效液相色谱图和总离子流图 1：灵芝酸I;2：灵芝烯酸C;3：灵芝酸C2;4：灵芝酸C6;5：灵芝酸G;6：灵芝烯酸B;7：灵芝酸B;8：灵芝烯酸A;9：灵芝酸K;10：灵芝酸A;11：灵芝酸H;12：赤芝酸A;13：灵芝酸N;14：灵芝烯酸H;15：灵芝烯酸D;16：灵芝酸D;17：灵芝酸F;18：灵芝酸DM;19：3-O-acetyl-16α-hydroxytra-metenolic acid. 下同

Fig. 1 UPLC and TIC graph of Ganoderma lingzhi triterpenoid standard. 1: Ganoderic acid I; 2: Ganoderenic acid C; 3: Ganoderic acid C2; 4: Ganoderic acid C6; 5: Ganoderic acid G; 6: Ganoderenic acid B; 7: Ganoderic acid B; 8: Ganoderenic acid A; 9: Ganoderic acid K; 10: Ganoderic acid A; 11: Ganoderic acid H; 12: Lucidenic acid A; 13: Ganoderic acid N; 14: Ganoderenic acid H; 15: Ganoderenic acid D; 16: Ganoderic acid D; 17: Ganoderic acid F; 18: Ganoderic acid DM; 19: 3-O-acetyl-16α-hydroxytra-metenolic acid. The same below.