基于1H-NMR及GC-MS的中国块菌及其共生云南松的代谢物分析

周雪,袁鹏宇,安霜,陈鹏至,陈幽幽,杨晓敏,李宗菊

Analyses of metabolites of Tuber sinense and its symbiotic Pinus yunnanensis based on 1H-NMR and GC-MS

Xue ZHOU,Pengyu YUAN,Shuang AN,Pengzhi CHEN,Youyou CHEN,Xiaomin YANG,Zongju LI

表2 中国块菌子实体及松根的GC-MS峰的指认

Table 2 Assignment of GC-MS peak of Tuber sinense fruiting bodies and Pinus yunnanensis roots

序号 No. 保留时间 RT (min) 代谢产物名称 Metabolites 分子式 Formula 分子量 m/z 相对峰面积 Relative peak area SL SG 1 3.037 Propanoic acid丙酸 C3H6O2 74.08 0.092±0.030 0.017±0.006 2 3.97 L-Valine缬氨酸 C5H11NO2 117.15 0.029±0.013 3 4.198 l-Alanine丙氨酸 C3H7NO2 89.09 0.059±0.032 4 4.44 Glycine甘氨酸 C2H5NO2 75.07 0.089±0.019 5 4.838 Ethanedioic acid乙二酸 H?C?O? 90.03 0.033±0.005 0.013±0.006 6 7.418 Serine丝氨酸 C3H7NO3 105.09 0.053±0.013 7 7.821 L-Leucine亮氨酸 C6H13NO2 131.17 0.006±0.003 8 8.44 l-Threonine苏氨酸 C4H9NO3 119.12 0.066±0.021 9 8.908 Butanedioic acid琥珀酸 C4H6O4 118.09 0.490±0.041 0.024±0.008 10 9.897 2-Butenedioic acid柠康酸 C5H6O4 130.1 0.092±0.010 11 11.641 l-Aspartic acid天门冬氨酸 C4H7NO4 133.10 0.008±0.004 12 15.805 L-Proline脯氨酸 C5H9NO2 115.13 0.152±0.013 13 15.935 Meso-Erythritol赤藓糖醇 C4H10O4 122.12 0.046±0.019 14 17.51 3-Pyridinecarboxylic acid烟酸 C6H5NO2 123.11 0.014±0.006 15 17.977 Pentanedioic acid戊二酸 C5H8O4 132.11 0.005±0.002 16 18.422 L-Asparagine天冬酰胺 C4H8N2O3 132.12 0.035±0.018 17 19.514 Glutamic acid谷氨酸 C5H9NO4 147.13 0.042±0.010 18 25.723 2-Keto-l-gluconic acid C6H10O7 194.14 0.079±0.005 2-酮基-D-葡萄糖酸 19 26.857 1,2,3-Propanetricarboxylic acid C6H8O6 176.12 1.741±0.864 1,2,3-丙三羧酸 20 27.185 Lysine赖氨酸 C6H14N2O2 146.19 0.036±0.010 21 28.837 D-(-)-Fructose果糖 C6H12O6 180.16 0.026±0.362 1.104±0.365 22 29.691 d-Glucose葡萄糖 C6H12O6 180.16 0.613±0.117 0.426±0.141 23 30.17 D-Allose阿洛糖 C6H12O6 180.15 0.111±2.784 24 32.395 Glucopyranose吡喃葡萄糖 C6H12O6 180.16 0.043±0.025 25 32.86 D-Gluconic acid葡萄糖酸 C6H12O7 196.16 0.012±0.006 0.015±0.005 26 33.042 Hexadecanoic acid棕榈酸 C16H32O2 256.42 0.032±0.005 0.032±0.020 27 38.103 Octadecanoic acid硬脂酸 C18H36O2 284.48 0.037±0.010 0.018±0.015 28 38.42 Glucitol山梨醇 C6H14O6 182.17 4.838±0.027 0.025±0.009 29 47.856 D-(+)-Trehalose海藻糖 C12H22O11 342.30 0.187±0.019 0.023±0.008 30 48.669 D-Myo-Inisitol肌醇 C6H12O6 180.16 1.262±0.004 31 5.501 Pentasiloxane十二甲基五硅氧烷 C12H36O4Si5 384.84 0.001±0.001 32 17.72 L-Threonic acid苏糖酸 C4H8O5 136.10 0.003±0.001 33 21.288 DL-Arabinose阿拉伯糖 C5H10O5 150.13 0.009±0.003 34 22.459 Levoglucosan内醚糖 C6H10O5 162.14 0.001±0.001 35 22.57 Xylitol木糖醇 C5H12O5 152.15 0.004±0.001 36 26.371 1-Cyclohexene-1-carboxylic acid C7H10O2 126.15 0.302±0.101 1-环己烯-1-甲酸 37 27.226 D-Pinitol松醇 C7H14O6 194.18 0.714±0.236 38 30.003 d-Mannose甘露糖 C6H12O6 180.16 0.073±0.025 39 30.447 D-Mannitol甘露醇 C6H14O6 182.17 0.022±0.007 40 35.078 Myo-Inositol肌醇 C6H12O6 180.16 0.133±0.045 41 35.709 2-Propenoic acid C15H28O2 240.38 0.009±0.003 正十一烷基甲基丙烯酸酯 42 44.695 3-α-Mannobiose甘露二糖 C12H22O11 342.40 0.050±0.017 43 46.29 β-Gentiobiose龙胆二糖 C12H22O11 342.30 0.052±0.017 44 50.194 Aucubin桃叶珊瑚苷 C15H22O9 346.33 0.016±0.004 45 44.057 D-(+)-Cellobiose纤维二糖 C12H22O11 342.30 0.006±0.004 46 46.214 α-D-Glucopyranoside吡喃葡萄糖苷 C26H44O8 484.63 0.028±0.006