单孢分离菌株的异核不对称特性揭示梯棱羊肚菌为假同宗结合真菌

柴红梅,马渊浩,刘萍,陈卫民,陶南,赵永昌

The asymmetrical distribution of opposite mating type nuclei in single-ascospore isolates revealed Morchella importuna is a pseudohomothallic fungus

CHAI Hongmei,MA Yuanhao,LIU Ping,CHEN Weimin,TAO Nan,ZHAO Yongchang

图2 PCR产物电泳图 A：单孢菌株交配基因PCR扩增产物. 1-23：23个单孢菌株分别扩增MAT1-1-1和MAT1-2-1交配基因的PCR产物;M1：DL2000 marker;CK：不加模板负对照. B：子囊果Y1-1、Y1-2、Y1-3菌柄菌盖组织交配基因PCR扩增产物. 1,5,9：菌柄组织的MAT1-1-1基因扩增产物;2,6,10：菌柄组织的MAT1-2-1基因扩增产物;3,7,11：菌盖组织的MAT1-1-1基因扩增产物;4,8,12：菌盖组织的MAT1-2-1基因扩增产物;M1：DL2000 marker. C：菌株YPL6-1、YPL6-3的MAT idiomorph PCR扩增产物. 1：菌株YPL6-1的MAT idiomorph 扩增产物;2：菌株YPL6-3的MAT idiomorph扩增产物;M2：DL10000 marker

Fig. 2 Agarose gel electrophoresis of the PCR products. A: Mating gene PCR products in the single ascospore strains. 1-23: Mating gene PCR products in 23 single ascospore strains; M1: DL2000 marker; CK: Negative control of no template. B: Mating gene PCR products in the stipe and pileus of ascocarps Y1-1, Y1-2 and Y1-3. 1, 5, 9: PCR products of MAT1-1-1 in the stipe; 2, 6, 10: PCR products of MAT1-2-1 in the stipe; 3, 7, 11: PCR products of MAT1-1-1 in the pileus; 4, 8, 12: PCR products of MAT1-2-1 in the pileus; M1: DL2000 marker. C: MAT idiomorph PCR products in the YPL6-1 and YPL6-3. 1: MAT idiomorph in the YPL6-1; 2: MAT idiomorph in the YPL6-3; M2: DL10000 marker.