分光光度法测定灵芝三萜含量的干扰因素探讨

程池露,汪旵,王金艳,唐传红,张劲松,冯娜,唐庆九

Discussion on interference factors in determination of triterpenes in Ganoderma lingzhi by spectrophotometric method

CHENG Chilu,WANG Chan,WANG Jinyan,TANG Chuanhong,ZHANG Jingsong,FENG Na,TANG Qingjiu

表2 灵芝酸类化合物的特征性官能团和测定值

Table 2 Structural functional group and measured values of ganoderic acids determined by spectrophotometric method

种类 Types 化合物 Compounds C3位官能团 Functional group on C3 双键位置 Position of double bond 测定值 Measured value (μg) 灵芝烯酸 Ganoderenic acids Ganoderenic acid B 3β-OH 8-9, 20-22 8.52±0.15 Ganoderenic acid D 3=O 8-9, 20-22 8.72±0.09 Ganoderenic acid C 3β-OH 8-9, 20-22 11.11±0.23 Ganoderenic acid F 3=O 8-9, 20-22 13.74±0.17 Ganoderenic acid A 3=O 8-9, 20-22 14.29±0.09 Ganoderenic acid E 3=O 8-9, 20-22 14.99±0.31 灵芝酸 (含有共轭双键) Ganoderic acids (Conjugated double bonds) Ganoderic acid TQ 3=O 7-8(9-11), 24-25 8.86±0.10 Ganoderic acid SZ 3=O 7-8(9-11), 24-25 9.77±0.23 Ganoderic acid Jb 3β-OH 7-8(9-11), 24-25 10.36±0.23 Ganoderic acid TN 3β-OH 7-8(9-11), 24-25 13.79±0.23 Ganoderic acid Y 3β-OH 7-8(9-11), 24-25 16.18±0.23 Ganoderic acid T1* 3α-OAc 7-8(9-11), 24-25 18.47±0.09 Ganoderic acid T* 3α-OAc 7-8(9-11), 24-25 18.47±0.09 Ganoderic acid Mk* 3α-OH 7-8(9-11), 24-25 18.87±0.09 Ganoderic acid Me* 3α-OAc 7-8(9-11), 24-25 19.42±0.15 Ganoderic acid P* 3α-OH 7-8(9-11), 24-25 20.49±0.29 Ganoderic acid TR 3=O 7-8(9-11), 24-25 22.10±0.15 Ganoderic acid R* 3α-OAc 7-8(9-11), 24-25 22.20±0.09 Ganoderic acid S* 3α-OH 7-8(9-11), 24-25 24.14±0.09 Lanosta-7,9(11),24-trien-3α-hydroxy-26-oic acid* 3α-OH 7-8(9-11), 24-25 41.91±0.09 灵芝酸 (不含共轭双键) Ganoderic acids (No conjugated double bonds) Ganoderic acid A 3=O 8-9 6.73±0.15 Ganoderic acid LM2 3=O 8-9, 24-25 7.63±0.15 Ganoderic acid N 3=O 8-9 7.78±0.15 Ganoderic acid C6 3β-OH 8-9 8.03±0.17 Ganoderic acid F 3=O 8-9 8.72±0.23 Ganoderic acid K 3β-OH 8-9 9.02±0.23 Ganoderic acid G 3β-OH 8-9 9.57±0.15 Ganoderic acid B 3β-OH 8-9 9.67±0.17 Ganoderic acid H 3β-OH 8-9 10.06±0.05 Ganoderic acid I 3β-OH 8-9 10.91±0.15 Ganoderic acid C2 3β-OH 8-9 11.01±0.09 Ganoderic acid L 3β-OH 8-9 11.21±0.15 12-Hydroxy-ganoderic acid D 3=O 8-9 11.21±0.20 Ganoderic acid DM 3=O 8-9, 24-25 12.35±0.31 20-Hydroxyganoderic acid G 3β-OH 8-9 12.75±0.23 Ganoderic acid D 3=O 8-9 13.50±0.09 7-Oxo-ganoderic acid Z 3β-OH 8-9, 24-25 16.38±0.09 23S-hydroxy-11,15-dioxo-ganoderic acid DM 3β-OH 8-9, 24-25 19.32±0.09 Ganoderic acid AM1 3β-OH 8-9 22.05±0.08 Ganoderic acid Z 3β-OH 8-9, 24-25 26.28±0.75