分光光度法测定灵芝三萜含量的干扰因素探讨

程池露,汪旵,王金艳,唐传红,张劲松,冯娜,唐庆九

Discussion on interference factors in determination of triterpenes in Ganoderma lingzhi by spectrophotometric method

CHENG Chilu,WANG Chan,WANG Jinyan,TANG Chuanhong,ZHANG Jingsong,FENG Na,TANG Qingjiu

表3 灵芝酮、醛、醇、内酯类化合物的特征性官能团和测定值

Table 3 Structural functional group and measured values of ganoderma ketones, aldehydes, alcohols, lactones determined by spectrophotometric method

化合物 Compounds C3位官能团 Functional group on C3 双键位置 Position of double bond 测定值 Measured value (μg) Ganoderiol A 3β-OH 7-8(9-11) 7.23±0.23 Ethyl ganoderate J 3=O 8-9 10.36±0.23 3β,7β,15β-Trihydroxy-11-oxo-lanosta-8-en-24->20 lactone 3β-OH 8-9 11.76±0.31 Ganoderlactone D 3β-OH 8-9 12.30±0.23 Ganolactone B 3β-OH 8-9 13.05±0.23 Lucialdehyde B 3=O 8-9, 24-25 13.65±0.38 Ganoderal A 3=O 7-8(9-11), 24-25 15.59±0.09 Ganodermanondiol* 3=O 7-8(9-11) 19.52±0.09 Ganoderone A 3β-OH 8-9, 24-25 21.46±0.31 Lucidal 3β-OH 8-9, 24-25 27.08±0.09 Ganodermanontriol* 3=O 7-8(9-11) 27.28±0.09 Lucialdehyde A 3β-OH 7-8(9-11), 24-25 30.31±0.26 Ganoderol A 3=O 7-8(9-11), 24-25 31.26±0.31 Ganoderiol F 3=O 7-8(9-11), 24-25 38.27±0.70 Ganoderol B* 3β-OH 7-8(9-11), 24-25 39.76±0.53