分光光度法测定灵芝三萜含量的干扰因素探讨

程池露,汪旵,王金艳,唐传红,张劲松,冯娜,唐庆九

Discussion on interference factors in determination of triterpenes in Ganoderma lingzhi by spectrophotometric method

CHENG Chilu,WANG Chan,WANG Jinyan,TANG Chuanhong,ZHANG Jingsong,FENG Na,TANG Qingjiu

表4 甾醇类化合物的特征性官能团和测定值

Table 4 Structural functional group and measured values of sterols determined by spectrophotometric method

化合物 Compounds C3位官能团 Functional group on C3 双键位置 Position of double bond 测定值 Measured value (μg) Ergosta-4,6,8(14),22-tetraen-3-one 3=O 4-5(6-7)(8-14), 22-23 7.71±0.17 β-Sitosterol 3β-OH 5-6 8.31±0.09 Cerevisterol 3β-OH 7-8, 22-23 16.04±0.10 Ergosterol peroxide 3β-OH 6-7, 22-23 20.45±0.12 3,5-Dihydroxyergosta-7,22-dien-6-one 3β-OH 7-8, 22-23 21.50±0.17 Ergosterol 3β-OH 5-6(7-8)22-23 21.79±0.10 3,5,9-Trihydroxyergosta-7,22-dien-6-one 3β-OH 7-8, 22-23 22.59±0.10 5,8-Epidioxyergosta6,9(11),22-trien-3-ol 3β-OH 6-7, 9-11, 22-23 22.71±0.03 6-O-Methylcerevisterol 3β-OH 7-8, 22-23 31.04±0.10 Stellasterol 3β-OH 7-8, 22-23 33.51±0.40