灰树花多糖对乳腺癌细胞MCF-7的凋亡作用

张文岭,赵飞飞,张兆波,杜国涛,赵丹,李兰梅

Breast cancer cell MCF-7 apoptosis induced by polysaccharides of Grifola frondosa

ZHANG Wenling,ZHAO Feifei,ZHANG Zhaobo,DU Guotao,ZHAO Dan,LI Lanmei

图2 PGF对MCF-7细胞凋亡的影响 A：四组MCF-7细胞Hoechst染色对比；B：流式细胞仪检测细胞凋亡情况；C：细胞数统计图. Negative表示活细胞数，Annexin V阳性表示细胞早期凋亡数，PI阳性表示死亡细胞数量，Annexin V与PI双阳性表示细胞晚期凋亡数量

Fig. 2 Effects of PGF on apoptosis of MCF-7 cells. A: Comparison of Hoechst staining of MCF-7 cells in four experimental groups; B: Cell apoptosis detected by flow cytometry; C: Statistical diagram of cell number. Negative indicated the number of living cells, Annexin V positive indicated the number of early apoptosis, PI positive indicated the number of dead cells, and Annexin V and PI double positive indicated the number of late apoptosis. Nor: Untreated cell; PGF20: 20 μg/mL; PGF50: 50 μg/mL; PGF100: 100 μg/mL.