灰树花多糖对乳腺癌细胞MCF-7的凋亡作用

张文岭,赵飞飞,张兆波,杜国涛,赵丹,李兰梅

Breast cancer cell MCF-7 apoptosis induced by polysaccharides of Grifola frondosa

ZHANG Wenling,ZHAO Feifei,ZHANG Zhaobo,DU Guotao,ZHAO Dan,LI Lanmei

图3 PGF对MCF-7细胞凋亡因子的调节 A：四组MCF-7细胞Western blotting蛋白表达水平；B：Bax蛋白表达水平灰度值统计图；C：Bcl-2蛋白表达水平灰度值统计图；D：Pro-Caspase-3蛋白表达水平灰度值统计图；E：Cleaved-Caspase-3蛋白表达水平灰度值统计图. *给药24 h后PGF不同给药浓度组与对照组的蛋白表达水平相比较差异显著(P<0.05)

Fig. 3 MCF-7 apoptosis factor regulated by PGF. A: Western blotting protein expression level of MCF-7 cells in four experimental groups; B: Gray scale of Bax protein expression level; C: Gray scale of Bcl-2 protein expression level; D: Statistical figure of the gray level of Pro-Caspase-3 protein expression; E: Gray level graph of Cleaved-Caspase-3 protein expression level. *After 24 h of treatment, the protein expression level of the experimental groups treated with different concentrations of PGF was compared with that of control group (P<0.05). Nor: Untreated cell; PGF20: 20 μg/mL; PGF50: 50 μg/mL; PGF100: 100 μg/mL.