pH调控临床光滑念珠菌生长的机制

刘静,陶丽,黄广华

Regulatory mechanism of pH on the growth of clinical Candida glabrata

Jing LIU,Li TAO,Guanghua HUANG

图5 突变体的获得 A：FDSG194 rim21-、FDSG194 rim8-及FDSG194 rim21-rim8-的基因敲除策略,其中SAT1和HIS3分别来源于质粒pSF2A和pSN52;B：FDSG194 rim21-、FDSG194 rim8-及FDSG194 rim21-rim8-突变体的验证. M：Marker;RIM21、rim21-：分别代表FDSG194的原始菌株和FDSG194 rim21-;RIM8、rim8-：分别代表FDSG194的原始菌株和FDSG194 rim8-;rim21-rim8-：代表FDSG194 rim21-rim8-. P1：RIM21 ORF F/R;P2：RIM8 ORF F/R;+：阳性对照组;-：阴性实验组

Fig. 5 Generation of gene deletion mutants of Candida glabrata. A: The deletion strategy for the RIM21 and RIM8 deletion mutants; SAT1 and HIS3 are derived from plasmids pSF2A and pSN52, respectively. B: The confirmation of FDSG194 rim21-, FDSG194 rim8- and FDSG194 rim21-rim8-. M: Marker; RIM21, rim21-: FDSG194 and FDSG194 rim21-; RIM8, rim8-: FDSG194 and FDSG194 rim8-; rim21-rim8-: FDSG194 rim21-rim8-. P1: RIM21 ORF F/R; P2: RIM8 ORF F/R; +: Positive control; -: Negative control.