HPLC测定灵芝子实体水提物及相关产品中三萜的含量

梁锐,冯娜,张劲松,李振皓,李明焱,张国亮,徐靖,王金艳,唐庆九

Determination of triterpenoids in Ganoderma lingzhi fruiting body water extract and related products by HPLC

Rui LIANG,Na FENG,Jinsong ZHANG,Zhenhao LI,Mingyan LI,Guoliang ZHANG,Jing XU,Jinyan WANG,Qingjiu TANG

表1 不同三萜类成分方法学数据

Table 1 Data of methodology of different triterpenoids

标准溶液 Standard samples 回归方程 Regressive equations 相关系数 Correlative coefficient 线性范围 Linear bounds (μg/mL) 相对校 正因子 Relative correction factors 精密度 Precision (%) 重复性 Repeatability (%) 稳定性 Stability (%) 平均回 收率 Recovery (%) 加样回收率相 对标准偏差 Relative standard deviation of sample recovery (%) 灵芝酸C2 Ganoderic acid C2 Y=8.97e+ 003X- 1.38e+003 0.9996 2.188-280 1.08 0.54 2.98 2.54 99.66 1.33 灵芝酸G Ganoderic acid G Y=6.08e+ 003X+ 1.84e+003 0.9996 2.656-340 1.59 0.20 1.36 1.49 100.24 0.45 灵芝烯酸B Ganoderenic acid B Y=2.24e+ 004X+ 3.35e+003 0.9994 0.781-100 0.43 0.02 0.02 2.43 99.01 1.71 灵芝酸B Ganoderic acid B Y=8.91e+ 003X- 1.07e+003 0.9996 2.188-280 1.08 0.01 0.15 0.12 100.13 1.00 灵芝酸A Ganoderic acid A Y=9.66e+ 003X- 2.39e+002 0.9996 1.953-250 1.00 0.16 0.96 0.25 101.46 1.58 灵芝酸H Ganoderic acid H Y=5.86e+ 003X+ 1.95e+003 0.9996 3.203-410 1.65 0.37 1.55 2.48 102.72 1.38 赤芝酸A Lucidenic acid A Y=5.12e+ 003X- 3.61e+002 0.9997 4.297-550 1.89 0.25 2.36 0.37 101.38 0.47 灵芝烯酸D Ganoderenic acid D Y=2.18e+ 004X+ 3.19e+002 0.9996 0.859-110 0.44 0.28 0.10 0.36 99.93 1.03 灵芝酸D Ganoderic acid D Y=1.03e+ 004X- 1.30e+003 0.9996 1.797-230 0.94 0.14 0.17 1.05 100.88 1.52 灵芝酸F Ganoderic acid F Y=7.77e+ 003X- 2.33e+002 0.9997 2.343-300 1.24 0.10 1.21 0.58 99.17 1.91