图1. 球孢白僵菌ZafA的特征及突变菌株的构建
A：序列结构特征;B：代表性丝状真菌ZafA蛋白的同源性比较,各分支上真菌学名后的括号内包括各同源序列的NCBI登录号以及与球孢白僵菌相应序列(粗体)的序列相似性;C：单基因敲除策略示意图;D：zafA突变株的PCR (1-3)和Southern杂交(4-6)鉴定. 1和4：野生株;2和5：敲除株;3和6：回补株

Fig. 1. Features of ZafA and construction of mutants in Beauveria bassiana.
A: Structural features of ZafA sequences; B: Phylogenetic tree of ZafA homologs in filamentous fungi constructed with a neighbor-joining method. The NCBI accession code of each homolog and its sequence identity (%) to the ZafA sequence of B. bassiana (bold) are parenthesized following each fungal name; C: Schematic diagram for zafA deletion; D: The mutants of zafA identified respectively via PCR (lanes 1-3) and Southern blotting (lanes 4-6). Lanes 1 and 4: Wild-type; Lanes 2 and 5: ΔzafA; Lanes 3 and 6: ΔzafA/zafA.