花溪盾孢囊霉：一个发现于中国贵阳的丛枝菌根真菌新种
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陈德瑶,龙春丽,何荣健,董芮豪,江龙
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Cetraspora huaxica, a new species of arbuscular mycorrhizal fungi (Glomeromycotina) from Guiyang, China
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CHEN Deyao,LONG Chunli,HE Rongjian,DONG Ruihao,JIANG Long
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表1 SSUmAf-LSUmAr与SSUmCf-LSUmBr引物信息
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Table 1 The primer information of SSUmAf-LSUmAr and SSUmCf-LSUmBr
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| Nested-PCR | 引物
Primers | 序列
Sequence (5?→3?) | PCR产物长度
Length of PCR product (bp) | 第一轮扩增
First amplification | SSUmAf1 | TGGGTAATCTTTTGAAACTTYA | 1 800 | | SSUmAf2 | TGGGTAATCTTRTGAAACTTCA | | LSUmAr1 | GCTCACACTCAAATCTATCAAA | | LSUmAr2 | GCTCTAACTCAATTCTATCGAT | | LSUmAr3 | TGCTCTTACTCAAATCTATCAAA | | LSUmAr4 | GCTCTTACTCAAACCTATCGA | | 第二轮扩增 | SSUmCf1 | TCGCTCTTCAACGAGGAATC | 1 500 | | Second amplification | SSUmCf2 | TATTGTTCTTCAACGAGGAATC | | SSUmCf3 | TATTGCTCTTNAACGAGGAATC | | LSUmBr1 | DAACACTCGCATATATGTTAGA | | LSUmBr2 | AACACTCGCACACATGTTAGA | | LSUmBr3 | AACACTCGCATACATGTTAGA | | LSUmBr4 | AAACACTCGCACATATGTTAGA | | LSUmBr5 | AACACTCGCATATATGCTAGA |
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