花溪盾孢囊霉：一个发现于中国贵阳的丛枝菌根真菌新种

陈德瑶,龙春丽,何荣健,董芮豪,江龙

Cetraspora huaxica, a new species of arbuscular mycorrhizal fungi (Glomeromycotina) from Guiyang, China

CHEN Deyao,LONG Chunli,HE Rongjian,DONG Ruihao,JIANG Long

图2 花溪盾孢囊霉形态特征 A-C：完整的孢子. D：PVLG中的破碎的孢子. 显示孢子壁层(OW和GW)，发芽盾(gs)，产孢细胞(Sc)，萌发管(gt). E，F：Melzer’s试剂中孢子壁层结构(OW+MW+IW). IWL3与Melzer’s试剂反应呈深粉色至亮红棕色. G：产孢细胞壁层结构(ScWL1-2)及“菌丝钉”结构(peg). H-J：发芽盾上具有一个中央胚芽孔(gp)，通常被大的折叠(f)隔开形成几个裂片，每个裂片上出现一个胚芽管起始点(gti). I，J：在PVLG溶液中用解剖针将发芽盾从孢子上剖离，且发芽盾壁(gsWL1-2)与Melzer’s试剂不发生反应. K，L：离体培养中的辅助细胞

Fig. 2 Microscopic morphological characteristics Cetraspora huaxica. A-C: Intact spores. D: Crushed spore on PVLG showing spore wall layers (OW and GW), germination shield (gs), a sporogenous cell (Sc), germinal tube (gt). E, F: In Melzer’s reagent, the spore wall layers (OW+MW+IW). IWL3 shows deep pink to bright red-brown reaction in Melzer’s reagent. G: Sporogenous cell wall layers (ScWL1-2) and ‘hyphal pegs’ structure. H-J: Germination shields (gs) with a initial central germ pore (gp), and several lobes that are generally separated by large folds (f); The lobes may regularly bear one germ tube initiation (gti). I, J: Independent germinated shields were completely separated from spores by tearing with a dissecting needle in PVLG liquid, and these shield wall layers (gsWL1-2) did not react in Melzer’s reagent. K, L: Auxiliary cells (aux) in root organ culture.