NaCl胁迫对白三叶生长及保护酶的影响

doi:10.11733/j.issn.1007-0435.2011.03.023

›› 2011, Vol. 19 ›› Issue (3): 492-496.DOI: 10.11733/j.issn.1007-0435.2011.03.023

NaCl胁迫对白三叶生长及保护酶的影响

徐威^1,2, 王瑜¹, 袁庆华¹

1. 中国农业科学院北京畜牧兽医研究所, 北京, 100193;
2. 山西农业大学, 山西, 太谷, 030801

收稿日期:2011-01-13 修回日期:2011-03-24 出版日期:2011-06-15 发布日期:2011-06-15
通讯作者: 袁庆华,E-mail:yuanqinghua@hotmail.com
作者简介:徐威(1986- ),男,河南省濮阳人,硕士研究生,研究方向为牧草种质资源,E-mail:xuwei1986777@sina.com
基金资助:
国家科技支撑项目(2011BAD17B01);国家牧草产业技术体系资助

Effect of NaCl on Growth and Activities of Protective Enzymes in Seedlings of White Clovers(Trifolium repens L.)

XU Wei^1,2, WANG Yu¹, YUAN Qing-hua¹

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China;
2. Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2011-01-13 Revised:2011-03-24 Online:2011-06-15 Published:2011-06-15

摘要/Abstract

摘要： 利用盆栽试验,对白三叶(Trifolium repens L.)2份耐盐品种1212和83-160以及2份敏盐品种83-159和81-1进行苗期耐盐性研究,通过对存活率、株高、电导率、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性等指标的测定与分析,探讨盐胁迫下白三叶生长情况及保护酶活性的响应。结果表明:随着盐浓度的升高,4个品种的存活率和相对株高均呈显著下降趋势,电导率呈上升趋势。耐盐品种1212和83-160的存活率和相对株高均显著高于敏盐品种81-1和83-159,电导率则显著低于敏盐品种,说明敏盐品种在盐胁迫下细胞受损更严重。随着胁迫时间的延长SOD,POD和CAT活性均表现为先急剧上升后下降之后再上升的趋势,耐盐品种和敏盐品种变化差异显著,在盐胁迫后5 d和10 d时,耐盐品种的3种酶活性低于敏盐品种,而盐胁迫后20 d时,耐盐品种酶活性和敏盐品种趋于一致,这可能是由于盐胁迫前期敏盐品种比耐盐品种中积累了更多的活性氧而使诱导酶活性提高。

关键词: 盐胁迫, 白三叶, 生长, 酶活性

Abstract: The effect of NaCl stress on growth and protective enzymatic activity of White Clover was investigated using seedling grown in pots.Two salt-tolerant varieties(1212,83-160) and two salt-sensitive varieties(83-159,81-1) with different NaCl stress(0%,1%,3%,5% and 7%) were studied here.The survival rate,plant height,electrolyte leakage rate,as well as the activity of Superoxide Dismutase(SOD),Peroxidase(POD) and Catalase(CAT) was tested.Results showed that the survival rate and relative plant height of these four varieties decreased with increased NaCl concentration,while the electrolyte leakage rate increased.The survival rate and relative plant height of salt-tolerant varieties(1212,83-160) were significantly higher than that of salt-sensitive varieties(83-159,81-1);while the electrolyte leakage rate of salt-tolerant varieties(1212,83-160) was significantly lower than that of salt-sensitive varieties(83-159,81-1).This suggests that the cells of salt-sensitive varieties were damaged more seriously under NaCl stress.With the extension of NaCl stress time,the activity of SOD,POD and CAT were all sharply increased,then declined,and then increased again.There are significant differences between salt-tolerant and salt-sensitive varieties.The activities of SOD,POD and CAT in salt-tolerant varieties were lower than salt-sensitive varieties after five to ten days of NaCl stress.The activities of SOD,POD and CAT in salt-tolerant and salt-sensitive varieties were no different after twenty days of NaCl stress.These results suggest that the accumulation of reactive oxygen increased the enzymatic activity in salt-sensitive varieties in the earlier stage of NaCl stress more than that in salt-tolerant varieties.

Key words: Salt-stress, White Clovers, Growth, Activities of protective enzymes

中图分类号:

徐威, 王瑜, 袁庆华. NaCl胁迫对白三叶生长及保护酶的影响[J]. , 2011, 19(3): 492-496.

XU Wei, WANG Yu, YUAN Qing-hua. Effect of NaCl on Growth and Activities of Protective Enzymes in Seedlings of White Clovers(Trifolium repens L.)[J]. , 2011, 19(3): 492-496.

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参考文献

[1] 殷秀杰,王明玖,石凤翎,等.三种三叶草幼苗期抗旱性差异的研究[J].中国草地学报,2008,30(2):68-73
[2] 翁森红,承斌.植物的耐盐性[J].中国草地,1995,17(4):70-72
[3] 任红旭,陈雄,王亚馥.抗旱性不同的小麦幼茁在水分和盐胁迫下抗氧化酶和多胺的变化[J].植物生态学报,2001,25(6):709-715
[4] 蒋玉蓉,吕有军,祝水金.棉花耐盐机理与盐害控制研究进展[J].棉花报,2006,18(4):248-254
[5] 张永峰,梁正伟,隋丽,等.盐碱胁迫对苗期紫花苜蓿生理特性的影响[J].草业学报,2009,18(4):230-235
[6] 王关林,李铁松,方宏筠,等.番茄转果聚糖合酶基因获得抗寒植株[J].中国农业科学,2004,37(8):1193-1197
[7] 李合生.植物生理生化实验原理和技术[M].高等教育出版社,2000.260-261
[8] 陈建勋,王晓峰.植物生理学实验指导.第二版[M].华南理工大学出版社,2006.72-73
[9] 吴欣明,王运琦,高洪文,等.羊茅属植物耐盐性评价及其对盐胁迫的生理反应[J].草业学报,2007,12(6):67-73
[10] Sreenivasulu N.Grimm B,Wobus U,et al.Differential response of antioxidant compounds to salinity stress in sahtolerant and salt-sensitive seedlings of foxtail millet(Setaria italica)[J].Physiologia Plantarum,2000,109:435-442
[11] Seandalios J G.Oxygen stress and superoxide dismutases[J].Plant Physiology,1993,101:7-12
[12] Hernandez J A,Ferrer M A,Jimenez,et al.Antioxidant systerns and O2/H2O2 produetion in the apoplast of pea leaves:its relation with salt-induced necrotic lesions in minor veins[J].Plant Physiology,2001,127:817-831
[13] Dionisio-Sese M L,Tobita S.Antioxidant responses of rice seedlings to salinity stress[J].Plant Science,1998,135:1-9
[14] Benavides M P,Marconi P L,Gallego S M,et al.Relationship between antioxidant defenee systems and salt tolerance in solasum tuborosum Australian[J].Plant Physiology,2000,27:273-278
[15] AD de Azevedo Neto,J T Prisco.Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes[J].Environmental and Experimental Botany,2006,56:87-94
[16] Rout N P,Shaw B P.Salt tolerance in aquatic maerophytes:possible involvement of the antioxidative enzymes[J].Plant Science,2001,160:415-423
[17] Amor N B,Jimenez A,Megdiche W,et al.Response of antioxidant systems to NaCl stress in the halophyte Cakile maritima[J].Physiologia Plantarun,2006,126:446-457
[18] Parida A K,Das A B,Mohanty P.Investigations on the antioxidative defence responses to NaCl stress in amangrove,Bruguiera parviflora:differential regulations of isoforms of some antioxidative enzymes[J].Plant Growth Regulation,2004,42:213-226
[19] 马丽,侯振安,梁永超,等.NaCl胁迫对棉花幼苗生理特性的影响[J].石河子大学学报,2008,26(4):180-184
[20] Breusegem F V,Vranova E,Dat J F,et al.The role of active oxygen species in plant signal transduction[J].Plant Science,2001,161:405-414

NaCl胁迫对白三叶生长及保护酶的影响

Effect of NaCl on Growth and Activities of Protective Enzymes in Seedlings of White Clovers(Trifolium repens L.)

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