海滨雀稗栽培品种的形态特征与AFLP分子标记分析

doi:10.11733/j.issn.1007-0435.2011.01.028

›› 2011, Vol. 19 ›› Issue (1): 164-170.DOI: 10.11733/j.issn.1007-0435.2011.01.028

海滨雀稗栽培品种的形态特征与AFLP分子标记分析

贺小霞, 刘一明, 王兆龙

上海交通大学农业与生物学院, 上海, 200240

收稿日期:2010-10-11 修回日期:2010-12-17 出版日期:2011-02-15 发布日期:2011-02-15
通讯作者: 王兆龙,E-mail:turf@sjtu.edu.cn
作者简介:贺小霞(1984- ),女,河南郑州人,硕士研究生,研究方向为草坪种质资源的分子检测,E-mail:xiami8262@sjtu.edu.cn
基金资助:
国家科技支撑计划(2006BAD01A19-3)资助

Morphological Characteristics and AFLP Analysis in Seashore Paspalum(Paspalum vaginatum) Varieties

HE Xiao-xia, LIU Yi-ming, WANG Zhao-long

School of Agriculture & Biology, Shanghai Jiaotong University, Shanghai 200240

Received:2010-10-11 Revised:2010-12-17 Online:2011-02-15 Published:2011-02-15

摘要/Abstract

摘要： 为解决海滨雀稗(Paspalum vaginatum)大面积营养扩繁过程中品种纯度问题,本研究利用AFLP分子标记技术,结合形态学特征,对海滨雀稗品种"Salam","SeaSpray","SeaDwarf","SeaIsle 2000"和一个"SeaDwarf"粗茎变异株系进行遗传差异分析,旨在探讨海滨雀稗品种的快速鉴别方法。结果表明:海滨雀稗的形态学遗传距离与AFLP分子标记相似系数的聚类结果一致,均表明Salam和SeaSpray之间的遗传差异较小,可以聚为一类,SeaD-warf和SeaIsle2000的亲缘关系较近,可以聚为另一类。另外,SeaDwarf粗茎变异株系与SeaDwarf之间的AFLP分子标记的遗传相似系数为0.5281,形态学遗传距离是4.8570,均表明二者之间遗传差异较大,无论是形态特征、还是AFLP分子标记均证实SeaDwarf粗茎株系确实已经变异。因此,AFLP分子标记的方法可用于海滨雀稗品种或者突变体的辅助鉴定。

关键词: 海滨雀稗, 粗茎变异株系, 形态学差异, AFLP分子标记

Abstract: Ensuring variety purity is the key issue for vegetative propagation in seashore paspalum(Paspalum vaginatum).Varietes "Salam","SeaSpray","SeaDwarf","SeaIsle 2000" and "SeaDwarf mutant" were analyzed by morphological and AFLP differences.Results show that AFLP analysis is similar to their morphological characteristics.Both indicate that "Salam" and "SeaSpray" are genetically closer and could be clustered in one group;"SeaDwarf" and "SeaIsle 2000" are in another group.AFLP analysis shows genetic similarity coefficients(GSCs) between "SeaDwarf" and "SeaDwarf mutant" is 0.5281;their morphological taxonomic dissimilarity(TD) is as high as 4.8570.All indicated that "SeaDwarf mutant" is a complete mutation from "SeaDwarf".Those results indicate that AFLP analysis could be used to assist the identification of seashore paspalum varieties or their mutants.

Key words: Seashore Paspalum, Thick-stem mutant, Morphological differences, AFLP Markers

中图分类号:

Q944-33
Q751

贺小霞, 刘一明, 王兆龙. 海滨雀稗栽培品种的形态特征与AFLP分子标记分析[J]. , 2011, 19(1): 164-170.

HE Xiao-xia, LIU Yi-ming, WANG Zhao-long. Morphological Characteristics and AFLP Analysis in Seashore Paspalum(Paspalum vaginatum) Varieties[J]. , 2011, 19(1): 164-170.

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海滨雀稗栽培品种的形态特征与AFLP分子标记分析

Morphological Characteristics and AFLP Analysis in Seashore Paspalum(Paspalum vaginatum) Varieties

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