草地学报

›› 2008, Vol. 16 ›› Issue (2): 121-124,140.DOI: 10.11733/j.issn.1007-0435.2008.02.004

• 研究论文 • 上一篇    下一篇

四脊裸胞壳Dh菌株侵染对钩麦蛾幼虫体内保护酶的影响

朱建兰1,2, 王国利1, 刘谨1, 张自和2   

  1. 1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 兰州, 730070;
    2. 兰州大学农业部草地农业生态系统重点开放实验室, 兰州, 730020
  • 收稿日期:2007-04-16 修回日期:2008-01-15 出版日期:2008-04-15 发布日期:2008-04-15
  • 作者简介:朱建兰(1956- ),女,宁夏中宁人,博士,教授,主要从事植物病理学及病虫害生物防治研究,Email:zhujl@gsau.edu.cn
  • 基金资助:
    甘肃省农牧厅生物技术专项(GNSW-2004-08)

The Protective Enzyme Activities of the Larva of Aproaerema anthylidella Hübner Infected by Emericella quadrilineata Dh Strain

ZHU Jian-lan1,2, WANG Guo-li1, LIU Jin1, ZHANG Zi-he2   

  1. 1. Pratacultural College, Gansu Agricultural University and Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu Province 730070, China;
    2. Key Laboratory of Grassland Agro-Ecosystems, Ministry of Agriculture and Lanzhou University, Lanzhou, Gansu Province 730020, China
  • Received:2007-04-16 Revised:2008-01-15 Online:2008-04-15 Published:2008-04-15

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摘要： 对四脊裸胞壳(Emericella quadrilineata)Dh菌株侵染苜蓿钩麦蛾(Aproaerema anthylidella Hübner)幼虫后其症状、体内保护酶活力进行研究,以期为害虫生物防治提供依据。结果表明:用菌悬液为5×107个/mL孢子处理,幼虫校正死亡率达88.38%;感病虫体表皮发黑,保湿后体壁受害部长出大量Dh菌株的菌丝和分生孢子;感病幼虫体内过氧化物酶(POD)活性和过氧化氢酶(CAT)活性呈先下降后上升趋势,但POD酶活性上升程度较CAT酶活性大;而超氧化物歧化酶(SOD)活性呈平稳上升趋势;从整体上看,用Dh菌株处理后钩麦蛾幼虫体内的3种保护酶的活性随时间的延长而呈上升的趋势。

关键词: Dh菌, 钩麦蛾, 超氧化物歧化酶(SOD), 过氧化氢酶(CAT), 过氧化物酶(POD)

Abstract: The entomopathogenic fungi,Emericella quadrilineata Dh strain,were isolated from the dead body of larva of Apocheima cinerarius Erschoff collected in field.The symptom and changes of protective enzymes activities of Aproaerema anthylidella Hübner larva infected by E.quadrilineata Dh strain were assayed.The results indicate that the correction death rate of the larvae was 88.38% when inoculated with the conidial suspension of Dh strain at the treating concentration of 5×107 spores/mL.The surface of cortices of the larva infected by Dh strain was black with lots of hyphae and conidia of the fungi on the epiderm of the illness larva after moist incubation.The POD and CAT activities of the larvae infected by Dh strain were significantly lower at the beginning and afterward higher than that of the control,but the speed of POD activity ascended relatively faster than that of CAT,and the SOD activity of the larvae went up smoothly in comparison to control.Generally,the activities of the above three preservative enzymes of the A.anthylidella worm infected E.quadrilineata Dh strain showed a rising trend along with the time proceeding.

Key words: Emericella quadrilineata(Dh), Aproaerema anthylidella Hübner, Superoxide dismutase(SOD), Catalase(CAT), Peroxidase(POD)

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