草地学报

›› 2007, Vol. 15 ›› Issue (5): 469-472.DOI: 10.11733/j.issn.1007-0435.2007.05.013

• 研究论文 • 上一篇    下一篇

空间飞行和60Co-γ射线辐照对苦荬菜种子的细胞学效应研究

刘丽1, 唐凤兰2, 张月学2, 韩微波2, 刘杰淋2, 蒿若超2, 徐香玲1, 李集临1   

  1. 1. 哈尔滨师范大学生命与环境科学学院生物系, 黑龙江, 哈尔滨, 150080;
    2. 黑龙江省农业科学院草业研究所, 黑龙江, 哈尔滨, 150086
  • 收稿日期:2007-05-14 修回日期:2007-05-30 出版日期:2007-10-15 发布日期:2007-10-15
  • 通讯作者: 张月学,E-mail:zyxnk y@163.com
  • 作者简介:刘丽(1982- ),女,黑龙江泰来人,硕士研究生,研究方向为牧草辐射诱变和生物技术育种

Cytological Effects of Aerospace Flight and 60Co-γ-Rays Irradiation on the Seeds of Lactuca indica L

LIU Li1, TANG Feng-lan2, ZHANG Yue-xue2, HAN Wei-bo2, LIU Jie-lin2, HAO Ruo-chao2, XU Xiang-ling1, LI Ji-lin1   

  1. 1. Biology Department, Life and Environment Science College, Harbin Normal University, Harbin, Heilongjiang Province 150080, China
    2. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China
  • Received:2007-05-14 Revised:2007-05-30 Online:2007-10-15 Published:2007-10-15

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摘要： 利用返回式卫星搭载苦荬菜(Lactuca indica)干种子,研究比较其与用不同剂量γ射线辐照对苦荬菜根尖细胞的遗传学效应。实验结果表明:与地面对照组相比,种子空间飞行可促进根尖细胞有丝分裂;而γ射线则抑制根尖细胞有丝分裂,并随着辐照剂量的增加有丝分裂指数逐步下降。二者均可诱发根尖细胞产生微核、染色体断裂、染色体粘连、落后染色体、游离染色体、桥等畸变。但空间飞行诱发畸变的频率明显低于γ射线辐照。在γ射线辐照剂量0～30Gy范围内,随着剂量的增加,单微核、多微核和染色体总畸变频率逐步升高。

关键词: 空间飞行, 60Co-γ射线, 有丝分裂指数, 核畸变, 染色体畸变, 苦荬菜

Abstract: The experiment was carried out to compare the cytological effects induced by aerospace and 60Co-γ-rays irradiation on the root tip cells of Lactuca indica L. seeds treated by recoverable satellite and irradiation at different dose 60Co-γ-rays in the range from 0 to 30Gy, respectively. The results show that aerospace flight stimulated the cell mitosis, but 60Co-γ-rays irradiation inhibited cell mitosis. With the increased dosage of 60Co-γ-rays irradiation, mitosis rate decreased. Both aerospace and 60Co-γ-rays irradiation induced various aberrations in root tip cells, such as micronucleus, chromosome fragment, chromosome conglutination, lagging chromosome, dissociative chromosome and bridge. However, the aberration rate caused by aerospace environment was lower than that caused by 60Co-γ-rays irradiation. The higher dosage of 60Co-γ-ray applied, the higher rates of single micronucleus、mutil-micronucleus, and total chromosome aberration occurred.

Key words: Space flight, 60Co-γ-rays, Mitotic index, Nuclear aberration, Chromosomal aberration, Lactuca indica L.

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